Overview

  • Product name

    Mouse NOV ELISA Kit (CCN3)
    See all NOV kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 8.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 5.9%
  • Sample type

    Cell culture supernatant, Plasma, Cell culture extracts, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    51 pg/ml
  • Range

    130 pg/ml - 8000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 111 107% - 116%
    Heparin Plasma 85 85% - 85%
    EDTA Plasma 88 86% - 88%
    Citrate Plasma 104 103% - 106%
    Mouse brain extract 113 108% - 117%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse, Rat
  • Product overview

    Abcam’s NOV (CCN3) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of NOV protein in mouse serum, plasma, cell and tissue extracts

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

    Sensitivity:
    Samples diluted in Sample Diluent NS: 95 pg/mL

    Samples diluted in Sample Diluent 75BS: 51 pg/mL

    Samples diluted in Cell Extraction Buffer PTR: 57 pg/mL

  • Notes

    NOV is a member of the CCN family of extracellular matrix associated signaling proteins. As a secreted protein, NOV regulates cell survival, adhesion, proliferation, differentiation and migration. In humans, NOV expression is associated with metastasis and poor prognosis.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse NOV Capture Antibody 1 x 600µl
    10X Mouse NOV Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BR 1 x 6ml
    Mouse NOV Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 75BS 1 x 20ml
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Function

    Immediate-early protein likely to play a role in cell growth regulation.
  • Tissue specificity

    Expressed in bone marrow, thymic cells and nephroblastoma. Increased expression in Wilms tumor of the stromal type.
  • Sequence similarities

    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • CCN family member 3
    • IBP-9
    • IGF-binding protein 9
    • IGFBP-9
    • Insulin-like growth factor-binding protein 9
    • Nephroblastoma-overexpressed gene protein homolog
    • NOV
    • NOV_HUMAN
    • NovH
    • Protein NOV homolog
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab205570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of mouse NOV were interpolated from the mouse NOV standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted. Interpolated values of NOV are plotted based on an extract load of 250 µg/mL.

  • Native NOV was measured in duplicate in 50% EDTA, heparin and citrate plasma diluted in Sample Diluent 75BS and concentrations interpolated from a standard curve diluted in Sample Diluent 75BS. The interpolated dilution factor corrected values are graphed (mean +/- SD).

Protocols

References

ab205570 has not yet been referenced specifically in any publications.

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