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  1. Link

    mouse-nse--neuron-specific-enolase-elisa-kit-ab233626.pdf

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Neuroscience Cell Type Marker Neuron marker Soma marker
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RecombinantSimpleStep

Mouse NSE / Neuron-specific Enolase ELISA Kit (ab233626)

  • Datasheet
  • SDS
  • Protocol Booklet
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Other - Mouse NSE / Neuron-specific Enolase ELISA Kit (ab233626)
  • Example of mouse Neuron-specific Enolase standard curve in Sample Diluent NS
  • Example of mouse Neuron-specific Enolase standard curve in 1X Cell Extraction Buffer PTR
  • Interpolated concentrations of native Neuron-specific Enolase in mouse brain tissue extract based on a 100 µg/mL extract load and Neuro-2a cell extract based on a 250 µg/mL extract load
  • Sandwich ELISA - Mouse NSE / Neuron-specific Enolase ELISA Kit (ab233626)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 50.8 pg/ml
  • Range: 312.5 pg/ml - 60000 pg/ml
  • Sample type: Cell culture extracts, Cell culture supernatant, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

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Overview

  • Product name

    Mouse NSE / Neuron-specific Enolase ELISA Kit
    See all NSE kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Mouse brain 5 7.1%
    Inter-assay
    Sample n Mean SD CV%
    Mouse brain 3 4.2%
  • Sample type

    Cell culture supernatant, Cell culture extracts, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    50.8 pg/ml
  • Range

    312.5 pg/ml - 60000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 101 97% - 107%
    Tissue Extracts 115 113% - 118%
    Cell culture media 89 92% - 98%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Mouse NSE / Neuron-specific Enolase ELISA Kit (ab233626) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NSE / Neuron-specific Enolase protein in tissue extracts, cell culture supernatant, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse NSE / Neuron-specific Enolase with 50.8 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse Neuron-specific Enolase Capture Antibody 1 x 600µl
    10X Mouse Neuron-specific Enolase Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Mouse Neuron-specific Enolase Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • Neuron Specific Enolase
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Neuroscience
    • Development
  • Function

    Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • Tissue specificity

    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Sequence similarities

    Belongs to the enolase family.
  • Developmental stage

    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Cellular localization

    Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • Target information above from: UniProt accession P09104 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • 2 phospho D glycerate hydrolyase
    • 2-phospho-D-glycerate hydro-lyase
    • Eno 2
    • ENO2
    • ENOG
    • ENOG_HUMAN
    • Enolase 2
    • Enolase 2 (gamma, neuronal)
    • Enolase 2 gamma neuronal
    • Enolase2
    • Epididymis secretory protein Li 279
    • Gamma enolase
    • Gamma-enolase
    • HEL S 279
    • Neural enolase
    • Neuron specific enolase
    • Neuron specific gamma enolase
    • Neuron-specific enolase
    • neuronal enriched enolase
    • Neurone specific enolase
    • NSE
    see all
  • Database links

    • Entrez Gene: 13807 Mouse
    • SwissProt: P17183 Mouse
    • Unigene: 3913 Mouse

    Associated products

    • Alternative Versions

      • Mouse NSE / Neuron-specific Enolase Antibody Pair - BSA and Azide free (ab244193)

    Images

    • Other - Mouse NSE / Neuron-specific Enolase ELISA Kit (ab233626)
      Other - Mouse NSE / Neuron-specific Enolase ELISA Kit (ab233626)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of mouse Neuron-specific Enolase standard curve in Sample Diluent NS
      Example of mouse Neuron-specific Enolase standard curve in Sample Diluent NS

      Background-subtracted data values (mean +/- SD) are graphed.

    • Example of mouse Neuron-specific Enolase standard curve in 1X Cell Extraction Buffer PTR
      Example of mouse Neuron-specific Enolase standard curve in 1X Cell Extraction Buffer PTR

      Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native Neuron-specific Enolase in mouse brain tissue extract based on a 100 µg/mL extract load and Neuro-2a cell extract based on a 250 µg/mL extract load
      Interpolated concentrations of native Neuron-specific Enolase in mouse brain tissue extract based on a 100 µg/mL extract load and Neuro-2a cell extract based on a 250 µg/mL extract load

      The concentrations of Neuron-specific Enolase were measured in duplicate and interpolated from the Neuron-specific Enolase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuron-specific Enolase concentration was determined to be 40,511 pg/mL in mouse brain tissue extract and 8,858 pg/mL in Neuro-2a cell extract.

    • Sandwich ELISA - Mouse NSE / Neuron-specific Enolase ELISA Kit (ab233626)
      Sandwich ELISA - Mouse NSE / Neuron-specific Enolase ELISA Kit (ab233626)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab233626? Please let us know so that we can cite the reference in this datasheet.

    ab233626 has been referenced in 2 publications.

    • Zhi LJ  et al. In situ amplified photothermal immunoassay for neuron-specific enolase with enhanced sensitivity using Prussian blue nanoparticle-loaded liposomes. Analyst 145:4164-4172 (2020). PubMed: 32369047
    • Wang J  et al. EGCG treats ICH via up-regulating miR-137-3p and inhibiting Parthanatos. Transl Neurosci 11:371-379 (2020). PubMed: 33335777

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