Overview

  • Product name

    Mouse NSE / Neuron-specific Enolase ELISA Kit
    See all NSE kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Mouse brain 5 7.1%
    Inter-assay
    Sample n Mean SD CV%
    Mouse brain 3 4.2%
  • Sample type

    Cell culture supernatant, Cell culture extracts, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    50.8 pg/ml
  • Range

    312.5 pg/ml - 60000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 101 97% - 107%
    Tissue Extracts 115 113% - 118%
    Cell culture media 89 92% - 98%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Human
  • Product overview

    Neuron-specific Enolase in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Neuron-specific Enolase protein in mousecell culture supernatants, cell and tissue extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Neuron-specific Enolase, also known as NSE, gamma-enolase and Enolase 2, is a cytoplasmic phosphopyruvate hydratase. Neuron-specific Enolase has two related family members, Enolase 1 and Enolase 3. Neuron-specific Enolase can be used to identify neuronal cells and normal or malignant cells with neuroendocrine origin.


    Sensitivity


    Samples diluted in Sample Diluent NS - 50.8 pg/mL


    Samples diluted in 1X Cell Extraction Buffer PTR - 160 pg/mL

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab233626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Neuron-specific Enolase were measured in duplicate and interpolated from the Neuron-specific Enolase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuron-specific Enolase concentration was determined to be 40,511 pg/mL in mouse brain tissue extract and 8,858 pg/mL in Neuro-2a cell extract.

Protocols

References

ab233626 has not yet been referenced specifically in any publications.

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