Mouse NSE / Neuron-specific Enolase ELISA Kit (ab233626)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 50.8 pg/ml
- Range: 312.5 pg/ml - 60000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse NSE / Neuron-specific Enolase ELISA Kit
See all NSE kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Mouse brain 5 7.1% Inter-assay Sample n Mean SD CV% Mouse brain 3 4.2% -
Sample type
Cell culture supernatant, Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
50.8 pg/ml -
Range
312.5 pg/ml - 60000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 101 97% - 107% Tissue Extracts 115 113% - 118% Cell culture media 89 92% - 98% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse NSE / Neuron-specific Enolase ELISA Kit (ab233626) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NSE / Neuron-specific Enolase protein in tissue extracts, cell culture supernatant, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse NSE / Neuron-specific Enolase with 50.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Neuron-specific Enolase Capture Antibody 1 x 600µl 10X Mouse Neuron-specific Enolase Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Mouse Neuron-specific Enolase Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. -
Tissue specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. -
Sequence similarities
Belongs to the enolase family. -
Developmental stage
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. -
Cellular localization
Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. - Information by UniProt
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Alternative names
- 2 phospho D glycerate hydrolyase
- 2-phospho-D-glycerate hydro-lyase
- Eno 2
see all -
Database links
- Entrez Gene: 13807 Mouse
- SwissProt: P17183 Mouse
- Unigene: 3913 Mouse
Associated products
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Alternative Versions
Images
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Other - Mouse NSE / Neuron-specific Enolase ELISA Kit (ab233626)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Neuron-specific Enolase standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Example of mouse Neuron-specific Enolase standard curve in 1X Cell Extraction Buffer PTRRaw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Neuron-specific Enolase in mouse brain tissue extract based on a 100 µg/mL extract load and Neuro-2a cell extract based on a 250 µg/mL extract loadThe concentrations of Neuron-specific Enolase were measured in duplicate and interpolated from the Neuron-specific Enolase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuron-specific Enolase concentration was determined to be 40,511 pg/mL in mouse brain tissue extract and 8,858 pg/mL in Neuro-2a cell extract.
References (1)
ab233626 has been referenced in 1 publication.
- Zhi LJ et al. In situ amplified photothermal immunoassay for neuron-specific enolase with enhanced sensitivity using Prussian blue nanoparticle-loaded liposomes. Analyst 145:4164-4172 (2020). PubMed: 32369047
