Mouse NSE / Neuron-specific Enolase ELISA Kit (ab233626)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse NSE / Neuron-specific Enolase ELISA Kit
See all NSE kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Mouse brain 5 7.1% Inter-assay Sample n Mean SD CV% Mouse brain 3 4.2% -
Sample type
Cell culture supernatant, Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
50.8 pg/ml -
Range
312.5 pg/ml - 60000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 101 97% - 107% Tissue Extracts 115 113% - 118% Cell culture media 89 92% - 98% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Predicted to work with: Human
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Product overview
Neuron-specific Enolase in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Neuron-specific Enolase protein in mousecell culture supernatants, cell and tissue extracts.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Neuron-specific Enolase, also known as NSE, gamma-enolase and Enolase 2, is a cytoplasmic phosphopyruvate hydratase. Neuron-specific Enolase has two related family members, Enolase 1 and Enolase 3. Neuron-specific Enolase can be used to identify neuronal cells and normal or malignant cells with neuroendocrine origin.
Sensitivity
Samples diluted in Sample Diluent NS - 50.8 pg/mL
Samples diluted in 1X Cell Extraction Buffer PTR - 160 pg/mL
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Neuron-specific Enolase Capture Antibody 1 x 600µl 10X Mouse Neuron-specific Enolase Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Mouse Neuron-specific Enolase Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. -
Tissue specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. -
Sequence similarities
Belongs to the enolase family. -
Developmental stage
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. -
Cellular localization
Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. - Information by UniProt
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Alternative names
- 2 phospho D glycerate hydrolyase
- 2-phospho-D-glycerate hydro-lyase
- Eno 2
see all -
Database links
- Entrez Gene: 2026 Human
- Entrez Gene: 13807 Mouse
- Omim: 131360 Human
- SwissProt: P09104 Human
- SwissProt: P17183 Mouse
- Unigene: 511915 Human
- Unigene: 3913 Mouse
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab233626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Mouse NSE / Neuron-specific Enolase ELISA Kit (ab233626)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Neuron-specific Enolase standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Example of mouse Neuron-specific Enolase standard curve in 1X Cell Extraction Buffer PTRRaw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Neuron-specific Enolase in mouse brain tissue extract based on a 100 µg/mL extract load and Neuro-2a cell extract based on a 250 µg/mL extract loadThe concentrations of Neuron-specific Enolase were measured in duplicate and interpolated from the Neuron-specific Enolase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuron-specific Enolase concentration was determined to be 40,511 pg/mL in mouse brain tissue extract and 8,858 pg/mL in Neuro-2a cell extract.
Protocols
References
ab233626 has not yet been referenced specifically in any publications.
