Mouse NUMB Matched Antibody Pair Kit (ab217617)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse NUMB Matched Antibody Pair Kit
See all NUMB kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse NUMB.
Both capture and detector antibodies are rabbit monoclonal antibodies.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website.
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab207614) which uses the same antibody pair.
To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.
Buffer information:
The capture antibody is glycerol free.
The detector antibody contains glycerol. -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 2 x 96 tests Mouse NUMB Capture Antibody 1 x 100µg 1 x 20µg Mouse NUMB Detector Antibody 1 x 25µg 1 x 5µg Mouse NUMB Lyophilized Protein 1 vial 1 vial -
Research areas
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Function
Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage. -
Sequence similarities
Contains 1 PID domain. -
Post-translational
modificationsIsoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- c14_5527
- C14orf41
- FLJ31314
see all -
Database links
- Entrez Gene: 18222 Mouse
- SwissProt: Q9QZS3 Mouse
- Unigene: 4390 Mouse
Associated products
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Alternative Versions
Images
Protocols
References
ab217617 has not yet been referenced specifically in any publications.