Overview

  • Product name

    Mouse P-Selectin ELISA Kit (CD62P)
    See all CD62P kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 5.6%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 3.21%
  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    4.16 pg/ml
  • Range

    23.4 pg/ml - 1500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 112 107% - 116%
    Cell culture media 119 118% - 120%
    Heparin Plasma 127 119% - 139%
    EDTA Plasma 122 119% - 126%
    Citrate Plasma 114 103% - 120%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rat, Rabbit, Goat, Hamster, Cow, Dog, Pig
  • Product overview

    Abcam’s P-Selectin (CD62P) in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of P-Selectin protein in serum, plasma, cell culture supernatants and cell extracts.

     The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

    Sensitivity: Samples diluted in Sample Diluent NS – 6.48 pg/mL

                         Samples diluted in Samples diluted in 1X Cell Extraction Buffer PTR – 4.16 pg/mL.

  • Notes

    P-Selectin is the largest member of the selectin family, which are adhesion molecules that ensure efficient recruitment of leukocytes to inflammation sites. It is stored in platelets and endothelial cells in Weibel-Palade bodies and α-granules, and is expressed by membrane translocation. P-Selectin’s main function is to mediate the interaction of activated endothelial cells or platelets with leukocytes, and most importantly, leukocyte rolling after trauma. High soluble levels of P-Selectin are thought to be predictive of future cardiovascular events.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse P-Selectin Capture Antibody 1 x 600µl
    10X Mouse P-Selectin Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CP 1 x 6ml
    Mouse P-Selectin Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
  • Tissue specificity

    Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
  • Involvement in disease

    Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • Sequence similarities

    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 9 Sushi (CCP/SCR) domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • Antigen CD62
    • CD62
    • CD62 antigen like family member P
    • CD62 antigen-like family member P
    • CD62P
    • FLJ45155
    • GMP 140
    • GMP-140
    • GMP140
    • Granule membrane protein 140
    • Granule membrane protein 140kDa
    • Granulocyte membrane protein
    • GRMP
    • LECAM3
    • Leukocyte endothelial cell adhesion molecule 3
    • Leukocyte-endothelial cell adhesion molecule 3
    • LYAM3_HUMAN
    • P selectin
    • P-selectin
    • PADGEM
    • Platelet activation dependent granule external membrane protein
    • Platelet activation dependent granule-external membrane protein
    • Platelet alpha granule membrane protein
    • PSEL
    • Selectin P
    • Selectin P (granule membrane protein 140kDa antigen CD62)
    • SELP
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab200014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Standard curve comparison between mouse P-Selectin (CD62P) SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 4-fold increase in sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The 1X dilution for serum and each plasma was at 2% matrix. The interpolated P-selectin concentrations multiplied by the total dilution factor are shown for each matrix (mean +/- SD, n = 2).

  • The 1X dilution for the lung extracts was 500 µg/mL, and for the spleen extracts 125 µg/mL. The interpolated P-Selectin concentrations multiplied by the total dilution factor are shown for each supernatant (mean +/- SD, n = 2).

Protocols

References

ab200014 has not yet been referenced specifically in any publications.

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