For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    mouse-pai1-total-elisa-kit-ab157529.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Blood Fibrinolysis / Thrombolysis
Share by email

Mouse PAI1 Total ELISA Kit (ab157529)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Biological samples for ab157529
  • Example Standard Curve for ab157529

Key features and details

  • Sensitivity: 0.006 ng/ml
  • Range: 0.05 ng/ml - 50 ng/ml
  • Sample type: Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse, Rabbit

You may also be interested in

Primary
Product image
Anti-FGF 23 antibody (ab123502)
ELISA
Product image
Mouse Plasminogen Total ELISA Kit (PLG) (ab198511)
Assay
Product image
Phosphate Assay Kit (Colorimetric) (ab65622)

View more associated products

Overview

  • Product name

    Mouse PAI1 Total ELISA Kit
    See all PAI1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 20 0.288ng/ml 0.023 7.9%
    2 20 1.64ng/ml 0.081 4.92%
    3 20 20.8ng/ml 1.86 8.95%
    Inter-assay
    Sample n Mean SD CV%
    1 10 0.307ng/ml 0.04 12.9%
    2 10 1.46ng/ml 0.155 10.6%
    3 10 16.6ng/ml 2.41 14.6%
  • Sample type

    Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.006 ng/ml
  • Range

    0.05 ng/ml - 50 ng/ml
  • Recovery

    100.8 %

    Sample specific recovery
    Sample type Average % Range
    Plasma 100.8 89.1% - 112%
  • Assay time

    1h 50m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse, Rabbit
  • Product overview

    Abcam’s Total PAI1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for is for the quantitative determination of active plasminogen activator inhibitor type 1 in mouse plasma. 

    Mouse PAI1 present in plasma reacts with the capture antibody coated and dried on a microtiter plate. Free, latent and complexed PAI1 will bind to the plate. Any unbound PAI1 is washed away and an anti-PAI1 primary antibody is added. Excess primary antibody is washed away and bound antibody, which is proportional to the total PAI1 present in the samples, is then reacted with the secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of total PAI1 in the sample.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer 1 x 50ml
    Anti-mouse PAI1 coated Microtiter Plate 1 unit
    Anti-mouse PAI1 Primary Antibody 1 vial
    Anti-rabbit horseradish peroxidase secondary antibody 1 x 10µl
    Mouse PAI1 Standard 1 vial
    TMB Substrate Solution 1 x 10ml
  • Research areas

    • Cardiovascular
    • Blood
    • Fibrinolysis / Thrombolysis
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Protease inhibitors
    • Serine protease inhibitors
    • SERPINs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Protease inhibitors
    • Other protease inhibitors
    • Cancer
    • Tumor biomarkers
    • Other
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Protease inhibitors ELISA kits
    • Metabolism
    • Types of disease
    • Metabolic disorders
  • Function

    This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • Tissue specificity

    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • Involvement in disease

    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • Sequence similarities

    Belongs to the serpin family.
  • Post-translational
    modifications

    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P05121 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Clade E
    • Endothelial plasminogen activator inhibitor
    • Nexin
    • Nexin plasminogen activator inhibitor type 1
    • PAI
    • PAI 1
    • PAI-1
    • PAI1_HUMAN
    • PLANH1
    • Plasminogen activator inhibitor 1
    • Plasminogen activator inhibitor type 1
    • Serine (or cysteine) proteinase inhibitor
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • Serine proteinase inhibitor clade E member 1
    • serpin
    • Serpin E1
    • Serpin peptidase inhibitor clade E
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • Serpine 1
    • SERPINE1
    see all
  • Database links

    • Entrez Gene: 18787 Mouse
    • SwissProt: P22777 Mouse
    • Unigene: 250422 Mouse

    Associated products

      Images

      • Biological samples for ab157529
        Biological samples for ab157529

        PAI1 measured in biological fluids showing quantity (ng) per mL of tested sample. Samples diluted 3-81 fold.

      • Example Standard Curve for ab157529
        Example Standard Curve for ab157529

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab157529? Please let us know so that we can cite the reference in this datasheet.

      ab157529 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab157529.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.