Overview

  • Product name
    Mouse PAI1 Total ELISA Kit
    See all PAI1 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    1 20 0.288ng/ml 0.023 7.9%
    2 20 1.64ng/ml 0.081 4.92%
    3 20 20.8ng/ml 1.86 8.95%
    Inter-assay
    Sample n Mean SD CV%
    1 10 0.307ng/ml 0.04 12.9%
    2 10 1.46ng/ml 0.155 10.6%
    3 10 16.6ng/ml 2.41 14.6%
  • Sample type
    Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.006 ng/ml
  • Range
    0.05 ng/ml - 50 ng/ml
  • Recovery

    100.8 %

    Sample specific recovery
    Sample type Average % Range
    Plasma 100.8 89.1% - 112%

  • Assay time
    1h 50m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rabbit
  • Product overview

    Abcam’s Total PAI1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for is for the quantitative determination of active plasminogen activator inhibitor type 1 in mouse plasma. 

    Mouse PAI1 present in plasma reacts with the capture antibody coated and dried on a microtiter plate. Free, latent and complexed PAI1 will bind to the plate. Any unbound PAI1 is washed away and an anti-PAI1 primary antibody is added. Excess primary antibody is washed away and bound antibody, which is proportional to the total PAI1 present in the samples, is then reacted with the secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of total PAI1 in the sample.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer 1 x 50ml
    Anti-mouse PAI1 coated Microtiter Plate 1 unit
    Anti-mouse PAI1 Primary Antibody 1 vial
    Anti-rabbit horseradish peroxidase secondary antibody 1 x 10µl
    Mouse PAI1 Standard 1 vial
    TMB substrate solution 1 x 10ml
  • Research areas
  • Function
    Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.
  • Tissue specificity
    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • Involvement in disease
    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • Sequence similarities
    Belongs to the serpin family.
  • Post-translational
    modifications
    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • Endothelial plasminogen activator inhibitor
    • PAI
    • PAI-1
    • PAI1
    • PAI1_HUMAN
    • PLANH1
    • Plasminogen activator inhibitor 1
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • Serpin E1
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • SERPINE1
    see all
  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab157529 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent concentration.

    Images

    • Biological samples for ab157529
      Biological samples for ab157529

      PAI1 measured in biological fluids showing quantity (ng) per mL of tested sample. Samples diluted 3-81 fold.

    • Example Standard Curve for ab157529
      Example Standard Curve for ab157529

    Protocols

    References

    ab157529 has not yet been referenced specifically in any publications.

    Publishing research using ab157529? Please let us know so that we can cite the reference in this datasheet.

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