Overview

  • Product name

    Mouse PCSK9 ELISA Kit
    See all PCSK9 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 2.4%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 8.4%
  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    2.5 pg/ml
  • Range

    55 pg/ml - 3500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98 88% - 105%
    Serum 104 102% - 107%
    Cell culture extracts 129 128% - 130%
    Tissue Extracts 102 99% - 105%
    Heparin Plasma 109 106% - 114%
    EDTA Plasma 101 101% - 102%
    Citrate Plasma 99 95% - 103%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rat, Rabbit, Cow, Human
  • Product overview

    PCSK9 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse PCSK9 in serum, plasmas, cell culture supernatants, cell and tissue extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:
    Samples in Sample Diluent NS: 2.5 pg/mL
    Samples in 1X Cell Extraction Buffer PTR: 12 pg/mL

  • Notes

    PCSK9 (proprotein convertase subtilisin kexin 9), also called proprotein convertase 9 or NARC-1 (neural apoptosis-regulated convertase 1), is a member of the proteinase K subfamily of subtilisin-related serine endoproteases. Mouse PCSK9 is encoded by the PCSK9 gene as a 694 amino acid enzyme that includes a signal peptide, a pro-domain, and a catalytic domain. PCSK9 undergoes autocatalytic cleavage in the endoplasmic reticulum to generate a 14 kDa prodomain and a 60 kDa catalytic domain. Active PCSK9 may undergo additional N-terminal proteolysis by furin or proprotein convertase 5/6A, creating an inactive 53 kDa form. PCSK9 is involved in the regulation of plasma cholesterol homeostasis by mediating the degradation of low density lipoprotein receptor.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (6 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab215538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of PCSK9 were measured in duplicate, interpolated from the PCSK9 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 0.25%, plasma (citrate) 0.25%, plasma (EDTA) 0.25%, plasma (heparin) 0.13% and RAW 264.7 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PCSK9 concentration was determined to be 353 ng/mL in serum, 396 ng/mL in plasma (citrate), 348 ng/mL in plasma (EDTA), and 495 ng/mL in plasma (heparin). Mouse PCSK9 was not detected in unspiked 50% RAW 264.7 cell culture supernatant.

  • The concentrations of PCSK9 were measured in duplicate, interpolated from the PCSK9 standard curve, and corrected for sample dilution. The interpolated, dilution-factor-corrected values are plotted (mean +/- SD, n=2). The mean native PCSK9 concentration was determined to be 2.36 ng/mg in liver extract and it was undetected in RAW 264.7 cell extract.

Protocols

References

ab215538 has not yet been referenced specifically in any publications.

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