Overview

  • Product name

    Mouse PD-1H ELISA Kit
    See all VISTA kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 9 2.9%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 2.5%
  • Sample type

    Serum, Tissue Extracts, Cell Lysate, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    44.3 pg/ml
  • Range

    390.63 pg/ml - 25000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 120 113% - 123%
    Tissue Extracts 111 103% - 118%
    Cell Lysate 106 102% - 110%
    Heparin Plasma 109 105% - 123%
    EDTA Plasma 117 108% - 123%
    Citrate Plasma 107 103% - 108%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rat, Cow, Human
  • Product overview

    PD-1H in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PD-1H protein in mouse serum, plasma, and cell and tissue extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Programmed Death-1 Homolog or PD-1H, also known as V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA), B7-H5, and Dies1, is a member of the immunoglobulin superfamily proteins and is encoded by the gene Vsir. PD-1H is a 308- amino acid type 1 glycosylated transmembrane protein that is primarily expressed in white blood cells and the transcription is partially controlled by p53. PD-1H has been shown to act as ligand and a receptor on T-cells to inhibit effector function. Increased PD-1H expression is related with an increase in immune activation and a decrease in CD4-posititive T-cells.


     

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BR 1 x 6ml
    10X Mouse PD-1H Capture Antibody 1 x 600µl
    10X Mouse PD-1H Detector Antibody 1 x 600µl
    Mouse PD-1H Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • B7 H5
    • B7H5
    • C10orf54
    • chromosome 10 open reading frame 54
    • DD1alpha
    • GI24
    • GI24_HUMAN
    • PD-1H
    • PDCD1 homolog
    • Platelet receptor Gi24
    • PP2135
    • sisp 1
    • SISP1
    • stress induced secreted protein 1
    • UNQ730/PRO1412
    • V domain Ig suppressor of T cell activation
    • V set domain containing immunoregulatory receptor
    • V set immunoregulatory receptor
    • V type immunoglobulin domain containing suppressor of T cell activation
    • VISTA
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab231933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • The PD-1H standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of PD-1H were measured in duplicates, interpolated from the PD-1H standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 100%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-1H concentration in the neat samples was determined to be 19.7 ng/mL in serum, 17.8 ng/mL in plasma (citrate), 15.3 ng/mL in plasma (EDTA), and 31.8 ng/mL in plasma (heparin).

  • The concentrations of PD-1H were measured in duplicate and interpolated from the PD-1H standard curve and corrected for sample dilution. Undiluted samples are 250 µg/mL for all extracts. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Protocols

References

ab231933 has not yet been referenced specifically in any publications.

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