Key features and details
- One-wash 90 minute protocol
- Sensitivity: 44.3 pg/ml
- Range: 390.63 pg/ml - 25000 pg/ml
- Sample type: Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse PD-1H ELISA Kit
See all VISTA kits
Intra-assay Sample n Mean SD CV% Serum 9 2.9% Inter-assay Sample n Mean SD CV% Serum 3 2.5%
Sample typeSerum, Tissue Extracts, Cell Lysate, Hep Plasma, EDTA Plasma, Cit plasma
Assay typeSandwich (quantitative)
Range390.63 pg/ml - 25000 pg/ml
Sample specific recovery Sample type Average % Range Serum 120 113% - 123% Tissue Extracts 111 103% - 118% Cell Lysate 106 102% - 110% Hep Plasma 109 105% - 123% EDTA Plasma 117 108% - 123% Cit plasma 107 103% - 108%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Mouse
Does not react with: Cow
Mouse PD-1H ELISA Kit (ab231933) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PD-1H protein in cit plasma, edta plasma, hep plasma, serum, tissue extracts, and cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PD-1H with 44.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Mouse PD-1H Capture Antibody 1 x 600µl 10X Mouse PD-1H Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse PD-1H Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
- Information by UniProt
- B7 H5
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
The PD-1H standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of PD-1H were measured in duplicates, interpolated from the PD-1H standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 100%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-1H concentration in the neat samples was determined to be 19.7 ng/mL in serum, 17.8 ng/mL in plasma (citrate), 15.3 ng/mL in plasma (EDTA), and 31.8 ng/mL in plasma (heparin).
The concentrations of PD-1H were measured in duplicate and interpolated from the PD-1H standard curve and corrected for sample dilution. Undiluted samples are 250 µg/mL for all extracts. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
ab231933 has not yet been referenced specifically in any publications.