Mouse PD1 ELISA Kit (ab210971)
- Datasheet
- References
- Protocols
Overview
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Product nameMouse PD1 ELISA Kit
See all PD1 kits -
Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% EL-4 ext 5 4% Inter-assay Sample n Mean SD CV% EL-4 ext 3 7% -
Sample typeCell culture supernatant, Cell culture extracts, Tissue Extracts
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Assay typeSandwich (quantitative)
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Sensitivity21 pg/ml
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Range125 pg/ml - 8000 pg/ml
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Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 100 93% - 104% Cell culture extracts 109 100% - 115% -
Assay time1h 30m
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Assay durationOne step assay
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Species reactivityReacts with: Mouse
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Product overview
PD1 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PD1 protein in mouse cell culture supernatant, cell and tissue extract samples.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Sensitivity:
Samples diluted in Sample Diluent NS MDD = 21 pg/mL
Samples diluted in 1X Cell Extraction Buffer PTR+Enhancer MDD = 33 pg/mL -
Notes
PD1 is a single-pass type I cell membrane glycoprotein. It is a receptor for PD-1L and/or PD-L2 ligands. PD1 is involved in the regulation of T-cell function during immunity and tolerance. PD-1 interactions with its ligands PD-L1 and/or PD-L2 provides a negative regulatory signal to CD4 and CD8 T cells that results ultimately in a phenotype termed T cell exhaustion. Possible cell death inducer, in association with other factors.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformPre-coated microplate (12 x 8 well strips)
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 10X Mouse PD-1 Capture Antibody 1 x 600µl 10X Mouse PD-1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Denaturant 1 x 500µl Mouse PD-1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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FunctionPossible cell death inducer, in association with other factors.
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Involvement in diseaseGenetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
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Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
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Developmental stageInduced at programmed cell death.
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Cellular localizationMembrane.
- Information by UniProt
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Alternative names
- CD279
- CD279 antigen
- hPD 1
see all -
Database links
- Entrez Gene: 18566 Mouse
- SwissProt: Q02242 Mouse
- Unigene: 5024 Mouse
Associated products
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Alternative Versions
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SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab210971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Mouse PD1 ELISA Kit (ab210971)ELISA Protocol Summary -
Example of mouse PD1 standard curve 1X Cell Extraction Buffer PTR+Enhancer.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse PD1 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native PD1 in mouse EL-4 cell extract samples based on a 180 µg/mL extract load.The concentrations of PD1 were measured in duplicate and interpolated from the PD1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD1 concentration was determined to be 8,606 pg/mL in EL-4 cell extract.
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Interpolated concentrations of native PD1 in PHA/PMA treated Day 2 EL-4 cell culture supernatant samples.The concentrations of PD1 were measured in duplicates, interpolated from the PD1 standard curves and corrected for sample dilution. Undiluted samples are as follows: EL-4 cell culture supernatant 100%. Dilutions beyond 4 fold were not analyzed due to O.D. values below the O.D. values of the MDD. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD1 concentration was determined to be 195 pg/mL in EL-4 cell culture supernatant samples.
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Comparison of PD1 in unstimulated and PHA/PMA (1.5% and 10ng/mL respectively) stimulated EL-4 supernatant.EL-4 cells were grown in the absence or presence of PHA/PMA for 48 hours. The concentrations of PD1 were measured in neat supernatant samples in duplicates and interpolated from the PD1 standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean PD1 concentration was determined to be 32 pg/mL in unstimulated, 203 pg/ml in PHA/PMA stimulated supernatants and undetectable in media (not shown).
Protocols
References
ab210971 has not yet been referenced specifically in any publications.
