SimpleStep

Mouse PD1 ELISA Kit (ab210971)

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Overview

  • Product name

    Mouse PD1 ELISA Kit
    See all PD1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    EL-4 ext 5 4%
    Inter-assay
    Sample n Mean SD CV%
    EL-4 ext 3 7%

  • Sample type

    Cell culture supernatant, Cell culture extracts, Tissue Extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    21 pg/ml

  • Range

    125 pg/ml - 8000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 100 93% - 104%
    Cell culture extracts 109 100% - 115%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse PD1 ELISA Kit (ab210971) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PD1 protein in cell culture extracts, cell culture supernatant, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PD1 with 21 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    PD1 is a single-pass type I cell membrane glycoprotein. It is a receptor for PD-1L and/or PD-L2 ligands. PD1 is involved in the regulation of T-cell function during immunity and tolerance. PD-1 interactions with its ligands PD-L1 and/or PD-L2 provides a negative regulatory signal to CD4 and CD8 T cells that results ultimately in a phenotype termed T cell exhaustion. Possible cell death inducer, in association with other factors.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse PD-1 Capture Antibody 1 x 600µl
    10X Mouse PD-1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPR 1 x 6ml
    Denaturant 1 x 500µl
    Mouse PD-1 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Possible cell death inducer, in association with other factors.

  • Involvement in disease

    Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.

  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Developmental stage

    Induced at programmed cell death.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Alternative names

    • CD279
    • CD279 antigen
    • hPD 1
    • hPD l
    • hPD-1
    • hSLE1
    • PD 1
    • PD-1
    • PD1
    • PDCD 1
    • PDCD1
    • PDCD1_HUMAN
    • Programmed cell death 1
    • Programmed cell death 1 protein
    • Programmed cell death protein 1
    • Protein PD 1
    • Protein PD-1
    • SLEB2
    • Systemic lupus erythematosus susceptibility 2
    see all

  • Database links

    Images

    • Other - Mouse PD1 ELISA Kit (ab210971)
      Other - Mouse PD1 ELISA Kit (ab210971)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of mouse PD1 standard curve 1X Cell Extraction Buffer PTR+Enhancer.
      Example of mouse PD1 standard curve 1X Cell Extraction Buffer PTR+Enhancer.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Example of mouse PD1 standard curve in Sample Diluent NS.
      Example of mouse PD1 standard curve in Sample Diluent NS.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native PD1 in mouse EL-4 cell extract samples based on a 180 µg/mL extract load.
      Interpolated concentrations of native PD1 in mouse EL-4 cell extract samples based on a 180 µg/mL extract load.

      The concentrations of PD1 were measured in duplicate and interpolated from the PD1 standard curve and corrected for sample dilution.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).  The mean PD1 concentration was determined to be 8,606 pg/mL in EL-4 cell extract.

    • Interpolated concentrations of native PD1 in PHA/PMA treated Day 2 EL-4 cell culture supernatant samples.
      Interpolated concentrations of native PD1 in PHA/PMA treated Day 2 EL-4 cell culture supernatant samples.

      The concentrations of PD1 were measured in duplicates, interpolated from the PD1 standard curves and corrected for sample dilution. Undiluted samples are as follows:  EL-4 cell culture supernatant 100%. Dilutions beyond 4 fold were not analyzed due to O.D. values below the O.D. values of the MDD. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).  The mean PD1 concentration was determined to be 195 pg/mL in EL-4 cell culture supernatant samples.

    • Comparison of PD1 in unstimulated and PHA/PMA (1.5% and 10ng/mL respectively) stimulated EL-4 supernatant.
      Comparison of PD1 in unstimulated and PHA/PMA (1.5% and 10ng/mL respectively) stimulated EL-4 supernatant.

      EL-4 cells were grown in the absence or presence of PHA/PMA for 48 hours. The concentrations of PD1 were measured in neat supernatant samples in duplicates and interpolated from the PD1 standard curve. The interpolated values are plotted (mean +/- SD, n=2).  The mean PD1 concentration was determined to be 32 pg/mL in unstimulated, 203 pg/ml in PHA/PMA stimulated supernatants and undetectable in media (not shown).

    Protocols

    References

    This product has been referenced in:

    • Su J  et al. Antitumor Activity of Extract From the Sporoderm-Breaking Spore of Ganoderma lucidum: Restoration on Exhausted Cytotoxic T Cell With Gut Microbiota Remodeling. Front Immunol 9:1765 (2018). Read more (PubMed: 30108589) »
    See 1 Publication for this product

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