Product nameMouse PDK4 Matched Antibody Pair Kit
See all PDK4 kits
Assay typeELISA set
Range125 pg/ml - 8000 pg/ml
Species reactivityReacts with: Mouse
Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse PDK4.
Both capture and detector antibodies are rabbit monoclonal antibodies.
Matched antibody pair kits and reagents deliver consistent, specific, and sensitive results.
- Batch-to-batch consistency: only recombinant monoclonal antibodies are used in our matched antibody pairs.
- Specificity: antibody pairs are screened in plasma and serum to ensure specificity in complex samples.
- Sensitivity: benchmarked against commercially available antibody pairs to ensure equivalent or superior performance compared with the competition.
Additional buffers and plates are required for the assay. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab215544) which uses the same antibody pair.
To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.
Tested applicationsSuitable for: ELISAmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 10 x 96 tests 2 x 96 tests Mouse PDK4 Capture Antibody 1 x 100µg 1 x 20µg Mouse PDK4 Detector Antibody 1 x 25µg 1 x 5µg Mouse PDK4 Lyophilized Protein 1 vial 1 vial
FunctionInhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Tissue specificityUbiquitous; highest levels of expression in heart and skeletal muscle.
Sequence similaritiesBelongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4
SimpleStep ELISA kits
Our Abpromise guarantee covers the use of ab218804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
ab218804 has not yet been referenced specifically in any publications.