Mouse Periostin ELISA Kit (ab193727)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse Periostin ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (qualitative) -
Sensitivity
0.05 ng/ml -
Range
0.05 ng/ml - 16 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 116.6 88% - 145% Plasma 109 84% - 134% Cell culture media 107 91% - 121% -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam's Periostin Mouse ELISA Kit (ab193727) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Periostin in serum, plasma (Plasma sample should be collected using EDTA or heparin as an anticoagulant. Citrate is not recommended.) and cell culture supernatants.
This assay employs an antibody specific for mouse Periostin coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Periostin present in the samples. The wells are washed and biotinylated anti-mouse Periostin antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Periostin bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated mouse Periostin detection antibody (lyophilized) 2 vials mouse Periostin Standards (lyophilized) 2 vials Pre-coated mouse Periostin Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Function
Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity). Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. -
Tissue specificity
Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. -
Sequence similarities
Contains 1 EMI domain.
Contains 4 FAS1 domains. -
Post-translational
modificationsGamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
Cellular localization
Golgi apparatus. Secreted, extracellular space, extracellular matrix. Colocalizes with BMP1 in the Golgi. - Information by UniProt
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Alternative names
- OSF-2
- Osteoblast-specific factor 2
- Periostin
see all -
Database links
- SwissProt: Q62009 Mouse
Images
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Example of typical Mouse Periostin standard curve using Assay dilutant A.Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Example of typical Mouse Periostin standard curve using Assay dilutant B.Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Protocols
References
ab193727 has not yet been referenced specifically in any publications.
