Mouse PF4 Matched Antibody Pair Kit (ab211763)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse PF4 Matched Antibody Pair Kit
See all PF4 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Range
7.81 pg/ml - 500 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse PF4. Both capture and detector antibodies are rabbit monoclonal antibodies.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website.
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab202403) which uses the same antibody pair.
To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.
Buffer information:
The capture antibody is glycerol free.
The detector antibody contains glycerol. -
Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 2 x 96 tests Mouse PF4 Capture Antibody 1 x 100µg 1 x 20µg Mouse PF4 Detector Antibody 1 x 25µg 1 x 5µg Mouse PF4 Lyophilized Protein 1 vial 1 vial -
Research areas
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Function
Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsBinds non-covalently to a proteoglycan molecule. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-X-C motif chemokine 4
- Chemokine (C X C motif) ligand 4
- Chemokine (CXC motif) ligand 4
see all -
Database links
- Entrez Gene: 56744 Mouse
- SwissProt: Q9Z126 Mouse
- Unigene: 332490 Mouse
Associated products
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Alternative Versions
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Matched antibody pairs
Applications
Our Abpromise guarantee covers the use of ab211763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. |
Images
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Standard calibration curve. Background subtracted values are graphed.
Protocols
References
ab211763 has not yet been referenced specifically in any publications.
