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    mouse-pf4-matched-antibody-pair-kit-ab211763.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Recombinant

Mouse PF4 Matched Antibody Pair Kit (ab211763)

  • Datasheet
  • SDS
  • Protocol Booklet
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Standard calibration curve. Background subtracted values are graphed.
  • Sandwich ELISA - Mouse PF4 Matched Antibody Pair Kit (ab211763)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Mouse
  • Range: 7.81 pg/ml - 500 pg/ml

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ELISA Accessory Pack (ab210905)
Protein
Product image
Recombinant mouse PF4 protein (Active) (ab243265)

View more associated products

Overview

  • Product name

    Mouse PF4 Matched Antibody Pair Kit
    See all PF4 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Sensitivity

    0.58 pg/ml
  • Range

    7.81 pg/ml - 500 pg/ml
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Mouse PF4 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse PF4.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab202403. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Tested applications

    Suitable for: ELISA, IAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 5 x 96 tests
    Mouse PF4 Capture Antibody 2 x 50µg 1 x 50µg
    Mouse PF4 Detector Antibody 2 x 12.5µg 1 x 12.5µg
    Mouse PF4 Lyophilized Protein 2 vials 1 vial
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cardiovascular
    • Blood
    • Platelets
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Chemokines
  • Function

    Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    Binds non-covalently to a proteoglycan molecule.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P02776 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • C-X-C motif chemokine 4
    • Chemokine (C X C motif) ligand 4
    • Chemokine (CXC motif) ligand 4
    • chemokine ligand 4
    • CXCL 4
    • CXCL4
    • Iroplact
    • MGC138298
    • Oncostatin A
    • Oncostatin-A
    • OncostatinA
    • PF 4
    • PF-4
    • PF4
    • Pf4a
    • Platelet factor 4
    • PLF4_HUMAN
    • SCYB 4
    • SCYB4
    • short form
    • Small inducible cytokine subfamily B
    • Small inducible cytokine subfamily member 4
    see all
  • Database links

    • Entrez Gene: 56744 Mouse
    • SwissProt: Q9Z126 Mouse
    • Unigene: 332490 Mouse

    Associated products

    • Alternative Versions

      • Mouse PF4 Antibody Pair - BSA and Azide free (ab241698)
    • Matched antibody pairs

      • Mouse PF4 ELISA Kit (CXCL4) (ab202403)
    • Related Products

      • ELISA Accessory Pack (ab210905)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab211763 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA
    Use at an assay dependent concentration.
    IA
    Use at an assay dependent concentration.
    Notes
    ELISA
    Use at an assay dependent concentration.
    IA
    Use at an assay dependent concentration.

    Images

    • Standard calibration curve. Background subtracted values are graphed.
      Standard calibration curve. Background subtracted values are graphed.
    • Sandwich ELISA - Mouse PF4 Matched Antibody Pair Kit (ab211763)
      Sandwich ELISA - Mouse PF4 Matched Antibody Pair Kit (ab211763)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab211763? Please let us know so that we can cite the reference in this datasheet.

    ab211763 has not yet been referenced specifically in any publications.

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