Overview

  • Product name

    Mouse Plasminogen Total ELISA Kit (PLG)
    See all Plasminogen kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Known Conc 20 8.4ng/ml 0.575 6.85%
    Known Conc 20 13.2ng/ml 0.884 6.72%
    Known Conc 20 150ng/ml 11.4 7.61%
    Inter-assay
    Sample n Mean SD CV%
    Known Conc 10 7.93ng/ml 0.736 9.29%
    Known Conc 10 14ng/ml 1.33 9.51%
    Known Conc 10 149ng/ml 8.76 5.87%
  • Sample type

    Urine, Serum, Plasma, Tissue Extracts, Cell culture media
  • Assay type

    Quantitative
  • Sensitivity

    0.38 ng/ml
  • Range

    1 ng/ml - 500 ng/ml
  • Recovery

    99.25 %

    Sample specific recovery
    Sample type Average % Range
    Spike = 99.5 96% - 103%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam's Plasminogen Total (PLG) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of total plasminogen in mouse plasma, serum, urine, cell culture media, or tissue extracts.

     

    Mouse plasminogen will bind to the capture antibody coated on the microtiter plate. Plasminogen, plasmin, and plasmin in complex with antiplasmin will react with the antibody on the plate. After appropriate washing steps, polyclonal anti-mouse plasminogen primary antibody binds to the plasminogen. Excess antibody is washed away and bound polyclonal antibody is then bound by the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of total plasminogen in the sample.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer 1 x 50ml
    Anti-mouse plasminogen primary antibody (lyophilized) 1 vial
    Anti-rabbit horseradish peroxidase secondary antibody 1 vial
    Mouse plasminogen standard (lyophilized) 1 vial
    PLG antibody pre-coated 96 well microplate (12 x 8 well strips) 1 x 96 tests
    TMB substrate solution 1 x 10ml
  • Research areas

  • Function

    Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
    Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.
  • Tissue specificity

    Present in plasma and many other extracellular fluids. It is synthesized in the liver.
  • Involvement in disease

    Defects in PLG are the cause of plasminogen deficiency (PLGD) [MIM:217090]. PLGD is characterized by decreased serum plasminogen activity. Two forms of the disorder are distinguished: type 1 deficiency is additionally characterized by decreased plasminogen antigen levels and clinical symptoms, whereas type 2 deficiency, also known as dysplasminogenemia, is characterized by normal, or slightly reduced antigen levels, and absence of clinical manifestations. Plasminogen deficiency type 1 results in markedly impaired extracellular fibrinolysis and chronic mucosal pseudomembranous lesions due to subepithelial fibrin deposition and inflammation. The most common clinical manifestation of type 1 deficiency is ligneous conjunctivitis in which pseudomembranes formation on the palpebral surfaces of the eye progresses to white, yellow-white, or red thick masses with a wood-like consistency that replace the normal mucosa.
  • Sequence similarities

    Belongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 5 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • Domain

    Kringle domains mediate interaction with CSPG4.
  • Post-translational
    modifications

    N-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
    In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide.
  • Cellular localization

    Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface.
  • Information by UniProt
  • Alternative names

    • Plasmin
    • Plasmin light chain B
    • PLG
    • PLMN_HUMAN
  • Database links

Applications

Our Abpromise guarantee covers the use of ab198511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Full range standard curve using ab198511 Plasminogen Total (PLG) Mouse ELISA Kit.

  • Linear range standard curve using ab198511 Plasminogen Total (PLG) Mouse ELISA Kit.

Protocols

References

ab198511 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab198511.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up