Key features and details
- Sensitivity: 1 pg/ml
- Range: 1.64 pg/ml - 400 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse PLGF-2 ELISA Kit (PGF)
Intra-assay Sample n Mean SD CV% overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 1 pg/ml
Range1.64 pg/ml - 400 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 110.3 104% - 116% Serum 95.3 87% - 103% Plasma 91.02 74% - 107%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Abcam’s PLGF-2 (PGF) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse PLGF-2 (PGF) in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse PLGF-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and PLGF-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse PLGF-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PLGF-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated anti-mouse PLGF-2 2 vials PLGF-2 Microplate (12 x 8 wells) 1 unit Recombinant Mouse PLGF-2 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
- SwissProt: P49764 Mouse
ab155459 has not yet been referenced specifically in any publications.