Overview

  • Product name

    Mouse PLTP ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Sample 1 16 9.4ng/ml 0.498 5.3%
    Sample 2 16 18.5ng/ml 0.907 4.9%
    Sample 3 16 27ng/ml 1.134 4.2%
    Inter-assay
    Sample n Mean SD CV%
    Sample 1 24 11.2ng/ml 0.75 6.7%
    Sample 2 24 17.9ng/ml 0.931 5.2%
    Sample 3 24 31ng/ml 1.49 4.8%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 20 pg/ml
  • Range

    0.78 ng/ml - 50 ng/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    The Mouse PLTP Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213883) is designed for the quantitative measurement of Mouse PLTP in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for PLTP has been precoated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: E18- A493) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLTP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse PLTP amount of sample captured in plate.

  • Notes

    Phospholipid transfer protein (PLTP), also known as lipid transfer protein II is a protein that in humans is encoded by the PLTP gene. This gene is mapped to 20q13.12. The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab213883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Mouse PLTP ELISA Kit (ab213883) Standard Curve.

Protocols

References

ab213883 has not yet been referenced specifically in any publications.

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