Mouse PLTP ELISA Kit (ab213883)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 0.78 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse PLTP ELISA Kit
See all PLTP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 1.418ng/ml 0.087 = 6.2% 2 16 4.309ng/ml 0.284 = 6.6% 3 16 22.189ng/ml 1.02 = 4.6% Inter-assay Sample n Mean SD CV% 1 24 1.296ng/ml 0.099 = 7.7% 2 24 4.212ng/ml 0.32 = 7.6% 3 24 21.284ng/ml 1.213 = 5.7% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 20 pg/ml -
Range
0.78 ng/ml - 50 ng/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
The Mouse PLTP Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213883) is designed for the quantitative measurement of Mouse PLTP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for PLTP has been precoated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: E18- A493) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLTP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse PLTP amount of sample captured in plate.
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Notes
Phospholipid transfer protein (PLTP), also known as lipid transfer protein II is a protein that in humans is encoded by the PLTP gene. This gene is mapped to 20q13.12. The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Mouse PLTP coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Mouse PLTP antibody 1 x 100µl Lyophilized recombinant Mouse PLTPstandard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Function
Converts HDL into larger and smaller particles. May play a key role in extracellular phospholipid transport and modulation of hdl particles. -
Tissue specificity
Wide tissue distribution. Placenta > pancreas > lung > kidney > heart > liver > skeletal muscle > brain. -
Sequence similarities
Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- BPI fold containing family E
- BPIFE
- HDLCQ9
see all -
Database links
- Entrez Gene: 18830 Mouse
- SwissProt: P55065 Mouse
- Unigene: 6105 Mouse
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab213883 has not yet been referenced specifically in any publications.