Overview

  • Product name

    Mouse PON2 ELISA Kit
    See all PON2 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Mouse spleen 5 3.2%
    Inter-assay
    Sample n Mean SD CV%
    Mouse spleen 3 4.1%
  • Sample type

    Cell culture extracts, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    41 pg/ml
  • Range

    78.13 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 95 93% - 99%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Product overview

    Abcam’s PON2 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PON2 protein in mouse cell and tissue extracts.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Serum Paraoxonase (PON2) is a 39-45 kDa, calcium binding, membrane-bound protein, ubiquitously expressed in mouse tissue. PON2 exhibits hydrolytic activity against acylhomoserine lactones, which are important bacterial quorum-sensing mediators, which might suggest a role in the defense response to pathogenic bacteria. Mutations of this gene have been suggested to be associated to vascular disease and with a number of quantitative phenotypes related to diabetes.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab208984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Undiluted liver, spleen, and NIH/3T3 extract is at 31,62.5, and 25 µg/mL extract, respectively The concentrations of mouse PON2 were measured in duplicate and interpolated from the mouse PON2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Interpolated values from the mouse PON2 protein standard are graphed. Undiluted Rat liver extract is at 12.5 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Protocols

References

ab208984 has not yet been referenced specifically in any publications.

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