Key features and details
- Sensitivity: 14 pg/ml
- Range: 10.97 pg/ml - 8000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse PPBP ELISA Kit (CXCL7)
See all CXCL7 kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 14 pg/ml
Range10.97 pg/ml - 8000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 123.5 108% - 133% Serum 118.3 100% - 135% Plasma 97.75 75% - 110%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Abcam’s PPBP Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse PPBP in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse PPBP coated on a 96- well plate. Standards and samples are pipetted into the wells and PPBP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse PPBP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PPBP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated anti-Mouse PPBP (lyophilized) 2 vials PPBP Microplate (12 x 8 wells) 1 unit Recombinant Mouse PPBP Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
RelevanceCXCL7 is a platelet-derived growth factor that belongs to the CXC chemokine family. It is a potent chemoattractant and activator of neutrophils and has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.
- B TG1
- Beta TG
- Beta thromboglobulin
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