Product nameMouse Prolactin ELISA Kit
See all Prolactin kits
Sample typeCell culture supernatant, Urine, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 30 pg/ml
Range27.43 pg/ml - 20000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 76.85 68% - 87% Serum 82.47 73% - 94% Plasma 76.78 67% - 85%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Abcam’s Prolactin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Prolactin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse Prolactin coated on a 96-well plate. Standards and samples are pipetted into the wells and Prolactin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Prolactin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Prolactin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Assay Diluent C 1 x 30ml Biotinylated anti-mouse Prolactin (lyophilized) 2 vials Prolactin Microplate (12 x 8 wells) 1 unit Recombinant Mouse Prolactin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
RelevanceVarious hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands. Prolactin acts primarily on the mammary gland by promoting lactation.
- Lactogenic Hormone
- Luteotropic Hormone
ab100736 has been referenced in 6 publications.
- Li C et al. Exposure to Organic Fraction Extracted from Oil Sands Process-Affected Water Has Negligible Impact on Pregnancy and Lactation of Mice. Environ Sci Technol 53:7083-7094 (2019). PubMed: 31117544
- Zhao C et al. Stromal Gli2 activity coordinates a niche signaling program for mammary epithelial stem cells. Science 356:N/A (2017). PubMed: 28280246
- Golub Y et al. Effects of In utero environment and maternal behavior on neuroendocrine and behavioral alterations in a mouse model of prenatal trauma. Dev Neurobiol 76:1254-1265 (2016). PubMed: 26899175
- O'Hara L et al. Pituitary androgen receptor signalling regulates prolactin but not gonadotrophins in the male mouse. PLoS One 10:e0121657 (2015). Mouse . PubMed: 25799562
- Iwama S et al. Pituitary expression of CTLA-4 mediates hypophysitis secondary to administration of CTLA-4 blocking antibody. Sci Transl Med 6:230ra45 (2014). PubMed: 24695685
- Stewart MK et al. The severity of mammary gland developmental defects is linked to the overall functional status of Cx43 as revealed by genetically modified mice. Biochem J 449:401-13 (2013). ELISA ; Mouse . PubMed: 23075222