Overview

  • Product name

    Mouse Prolactin ELISA Kit
    See all Prolactin kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 30 pg/ml
  • Range

    27.43 pg/ml - 20000 pg/ml
  • Recovery

    81 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 76.85 68% - 87%
    Serum 82.47 73% - 94%
    Plasma 76.78 67% - 85%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s Prolactin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Prolactin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse Prolactin coated on a 96-well plate. Standards and samples are pipetted into the wells and Prolactin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Prolactin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Prolactin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Assay Diluent C 1 x 30ml
    Biotinylated anti-mouse Prolactin (lyophilized) 2 vials
    Prolactin Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse Prolactin Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Relevance

    Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands. Prolactin acts primarily on the mammary gland by promoting lactation.
  • Cellular localization

    Secreted
  • Alternative names

    • AV290867
    • Lactogenic Hormone
    • Luteotropic Hormone
    • Mammotropin
    • OTTMUSP00000000651
    • PRL
    • Prl1a1
    • Prol
    • Prolactin precursor
    • RATPRLSD1
    • RNPROL
    • RP23-351I6.2
    • RP3-404K8.1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Prolactin measured in mouse biological fluids showing quantity (pg) per mL of tested sample. Samples diluted 10-270 fold.

  • Representative standard curve using ab100736

  • Representative standard curve using ab100736

Protocols

References

This product has been referenced in:

  • Golub Y  et al. Effects of In utero environment and maternal behavior on neuroendocrine and behavioral alterations in a mouse model of prenatal trauma. Dev Neurobiol 76:1254-1265 (2016). Read more (PubMed: 26899175) »
  • O'Hara L  et al. Pituitary androgen receptor signalling regulates prolactin but not gonadotrophins in the male mouse. PLoS One 10:e0121657 (2015). Mouse . Read more (PubMed: 25799562) »
See all 4 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

To determine the prolactin (PRL) contents of the pituitary gland, we extracted PRL from the pituitary gland of mice on 19th day of gestation by using 1 M urea/PBS solution. PRL levels were measured by using a ELISA kit (ab100736, lot no. GR291799-1 Abcam).
We detected the pituitary PRL contents with this ELISA kit.
Moreover, when the anterior pituitaries were homogenized and frozen-thawed in PBS (pH 7) containing 1 M urea, yields of PRL was maximum rather than in PBS only. This procedure would be the best for the estimation of PRL.

Sir or Madam シモカワ

Verified customer

Submitted Feb 08 2018

Question
Answer



Wir empfehlen eine 5- 100 fache Verdünnung für Serumproben. Da der Level an Zielprotein sehr variieren kann zwischen verschiedenen Proben muss der optimale Verdünnungsfaktor experimentell vom Benutzer herausgefunden werden. Wir empfehlen eine Verdünnungsreihe zu testen.

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1126552.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Question
Answer

Thank you for contacting us. I apologize for any inconvenience.

I can confirm that the spiky bottomed vial is Item-F (Biotinylated anti mouse antibody) and the round bottomed vials Item C are recombinant prolacticn.

Please let me know if the kit does not work, I will be happy to assist further.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

I am sorry you received a kit which contained a contaminated buffer and for any trouble this has caused. As requested, I have issued a free of charge replacement for one kit of ab100736.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Answer

Unless there is some discoloration I don’t think you have much to worry about especially if the standard curve looks fine as it is likely just precipitation. Do you have a photo you could send me?

As the diluent does contain proteins and other solutes it would not be abnormal to see a little cloudiness or precipitates, especially after being stored at a low temperature and/or for a long period of time. Most of the time bringing the diluent up to room temperature, mixing it well and then diluting it to its 1X working concentration will help incorporate any protein aggregates.

Please let me know if warming and mixing the diluentdoes not dissolve the large chunks.

Read More

Answer

Thank you for your inquiry. Please see my answers to your questions below: 1. I can confirm that there are special Serum collection tubes commercially available from many vendors. But serum can also easily be extracted by your customer by hand. Take blood and incubate without addition of any reagents for 2 hours at 37C or over night at 4C. This will lead to coagulation of the blood. Spin down the vials and take the clear, cell-free supernatant which is the serum. 2. I can recommend serum to be stored at -20C. 3. I suggest to start with a serum dilution of 1:10 for the first test with this kit. I recommend to always at least run duplicate samples. The optimal dilution has to be determined by the user experimentally. I hope this information was helpful. Please feel free to contact me again with any additional questions.

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