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    mouse-prolactinprl-elisa-kit-ab214572.pdf

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RecombinantSimpleStep

Mouse Prolactin/PRL ELISA Kit (ab214572)

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  • SDS
  • Protocol Booklet
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Other - Mouse Prolactin/PRL ELISA Kit (ab214572)
  • Example of mouse Prolactin standard curve in Sample Diluent NS.
  • Example of mouse Prolactin standard curve in1X Cell Extraction Buffer PTR.
  • Interpolated concentrations of spike Prolactin in normal mouse serum samples.
  • Interpolated concentrations of native Prolactin in mouse pituitary cell extract based on a 250 ng/mL extract load.
  • Comparison of native Prolactin concentrations in pregnant and normal mouse serum.
  • Sandwich ELISA - Mouse Prolactin/PRL ELISA Kit (ab214572)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 139 pg/ml
  • Range: 0.562 ng/ml - 36 ng/ml
  • Sample type: Cell culture extracts, Serum, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

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Overview

  • Product name

    Mouse Prolactin/PRL ELISA Kit
    See all Prolactin/PRL kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 9%
  • Sample type

    Serum, Cell culture extracts, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    139 pg/ml
  • Range

    0.562 ng/ml - 36 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 75 69% - 80%
    Tissue Extracts 102 98% - 109%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Cow
  • Product overview

    Mouse Prolactin/PRL ELISA Kit (ab214572) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Prolactin/PRL protein in cell culture extracts, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Prolactin/PRL with 139 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Prolactin/PRL is a secreted neuroendocrine pituitary hormone. Prolactin acts primarily on the mammary gland by promoting lactation, but has also additional pleiotropic effects both in males and females. Mature prolactin is a 199 amino acid long secreted monomeric protein. Prolactin can be also found in less biologically active form, macroprolactin, in a complex with IgG.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    10X Mouse Prolactin Capture Antibody 1 x 600µl
    10X Mouse Prolactin Detector Antibody 1 x 600µl
    Mouse Prolactin Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
  • Relevance

    Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands.
  • Cellular localization

    Secreted
  • Alternative names

    • Decidual prolactin
    • GHA1
    • Growth hormone A1
    • Lactogenic hormone
    • Luteotropic hormone
    • Mammotropin
    • PRL
    • Prolactin
    • Prolactin precursor
    see all
  • Database links

    • Entrez Gene: 19109 Mouse
    • SwissProt: P06879 Mouse
    • Unigene: 1270 Mouse

    Associated products

      Images

      • Other - Mouse Prolactin/PRL ELISA Kit (ab214572)
        Other - Mouse Prolactin/PRL ELISA Kit (ab214572)

        SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

         

      • Example of mouse Prolactin standard curve in Sample Diluent NS.
        Example of mouse Prolactin standard curve in Sample Diluent NS.

        Background-subtracted data values (mean +/- SD) are graphed.

      • Example of mouse Prolactin standard curve in1X Cell Extraction Buffer PTR.
        Example of mouse Prolactin standard curve in1X Cell Extraction Buffer PTR.

        Background-subtracted data values (mean +/- SD) are graphed.

      • Interpolated concentrations of spike Prolactin in normal mouse serum samples.
        Interpolated concentrations of spike Prolactin in normal mouse serum samples.

        The concentrations of Prolactin were measured in duplicates, interpolated from the Prolactin standard curves and corrected for sample dilution. Undiluted samples are as follows: normal mouse serum 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

      • Interpolated concentrations of native Prolactin in mouse pituitary cell extract based on a 250 ng/mL extract load.
        Interpolated concentrations of native Prolactin in mouse pituitary cell extract based on a 250 ng/mL extract load.

        The concentrations of Prolactin were measured in duplicate and interpolated from the Prolactin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prolactin concentration was determined to be 20 ng/mL in mouse pituitary cell extract.

      • Comparison of native Prolactin concentrations in pregnant and normal mouse serum.
        Comparison of native Prolactin concentrations in pregnant and normal mouse serum.

        The concentrations of Prolactin were measured in three 2-fold serial dilutions starting at 25% serum samples in duplicates, interpolated from the Prolactin standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng of Prolactin per mL of neat serum (mean +/- SD, n=2). The mean Prolactin concentration was determined to be 6.5 ng/mL in pregnant mouse serum and 2.6 ng/mL in normal mouse serum.

      • Sandwich ELISA - Mouse Prolactin/PRL ELISA Kit (ab214572)
        Sandwich ELISA - Mouse Prolactin/PRL ELISA Kit (ab214572)
        To learn more about the advantages of recombinant antibodies see here.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab214572? Please let us know so that we can cite the reference in this datasheet.

      ab214572 has not yet been referenced specifically in any publications.

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