Mouse Prolactin/PRL ELISA Kit (ab214572)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse Prolactin/PRL ELISA Kit
See all Prolactin/PRL kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 6% Inter-assay Sample n Mean SD CV% Overall 3 9% -
Sample type
Serum, Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
139 pg/ml -
Range
0.562 ng/ml - 36 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 75 69% - 80% Tissue Extracts 102 98% - 109% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Prolactin/PRL in vitro SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Prolactin protein in mouse serum and cell and tissue extract samples.
The SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Sensitivity:
Samples in Sample Diluent NS: 139 pg/mL
Samples in 1X Cell Extraction Buffer PTR: 168 pg/mL
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Notes
Prolactin/PRL is a secreted neuroendocrine pituitary hormone. Prolactin acts primarily on the mammary gland by promoting lactation, but has also additional pleiotropic effects both in males and females. Mature prolactin is a 199 amino acid long secreted monomeric protein. Prolactin can be also found in less biologically active form, macroprolactin, in a complex with IgG.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml 10X Mouse Prolactin Capture Antibody 1 x 600µl 10X Mouse Prolactin Detector Antibody 1 x 600µl Mouse Prolactin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands. -
Cellular localization
Secreted -
Alternative names
- Decidual prolactin
- GHA1
- Growth hormone A1
see all -
Database links
- Entrez Gene: 19109 Mouse
- SwissProt: P06879 Mouse
- Unigene: 1270 Mouse
Images
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Other - Mouse Prolactin/PRL ELISA Kit (ab214572)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Prolactin standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse Prolactin standard curve in1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spike Prolactin in normal mouse serum samples.The concentrations of Prolactin were measured in duplicates, interpolated from the Prolactin standard curves and corrected for sample dilution. Undiluted samples are as follows: normal mouse serum 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native Prolactin in mouse pituitary cell extract based on a 250 ng/mL extract load.The concentrations of Prolactin were measured in duplicate and interpolated from the Prolactin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prolactin concentration was determined to be 20 ng/mL in mouse pituitary cell extract.
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Comparison of native Prolactin concentrations in pregnant and normal mouse serum.The concentrations of Prolactin were measured in three 2-fold serial dilutions starting at 25% serum samples in duplicates, interpolated from the Prolactin standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng of Prolactin per mL of neat serum (mean +/- SD, n=2). The mean Prolactin concentration was determined to be 6.5 ng/mL in pregnant mouse serum and 2.6 ng/mL in normal mouse serum.
Protocols
References
ab214572 has not yet been referenced specifically in any publications.
