Key features and details
- Range: 0.5 ng/ml - 100 ng/ml
- Sample type: Cell culture media
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Mouse
Product nameMouse Prorenin + renin ELISA kit
Sample typeCell culture media
Range0.5 ng/ml - 100 ng/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Mouse Prorenin + renin ELISA kit (ab272777) is intended for the quantitative determination of total prorenin and renin antigen in cell culture media samples.
Mouse prorenin and renin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled polyclonal anti-mouse prorenin primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is reacted with streptavidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse prorenin. Color development is proportional to the total concentration of prorenin and renin in the samples.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-Mouse Prorenin + renin Primary Antibody Lyophilized Vial 1 vial Mouse Prorenin + renin ELISA Plate 1 unit Mouse Prorenin + renin Standard Lyophilized Vial 1 vial Streptavidin-HRP Secondary Reagent 1 vial TMB Substrate 1 x 10ml
ab272777 has not yet been referenced specifically in any publications.