• Product name

    Mouse Prostasin ELISA Kit
    See all PRSS8 kits
  • Detection method

  • Precision

    Sample n Mean SD CV%
    Sample 1 16 1.45ng/ml 0.08 5.6%
    Sample 2 16 25.2ng/ml 1.06 4.2%
    Sample 3 16 38.1ng/ml 2.02 5.3%
    Sample n Mean SD CV%
    Sample 1 24 2.64ng/ml 0.19 7.2%
    Sample 2 24 18.5ng/ml 1.18 6.4%
    Sample 3 24 40.7ng/ml 3.5 8.6%
  • Sample type

    Cell culture supernatant, Urine, Serum, Heparin Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 50 pg/ml
  • Range

    0.78 ng/ml - 50 ng/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    The Mouse Prostasin Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213884) is designed for the quantitative measurement of Mouse Prostasin in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for Prostasin has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: A30-Q289) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Prostasin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Prostasin amount of sample captured in plate.

  • Notes

    Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60% to 80% increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)


  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Mouse Prostasin coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Mouse Prostasin antibody 1 x 130µl
    Lyophilized recombinant Mouse Prostasin standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Possesses a trypsin-like cleavage specificity.
  • Tissue specificity

    Found in prostate, liver, salivary gland, kidney, lung, pancreas, colon, bronchus and renal proximal tubular cells. In the prostate gland it may be synthesized in epithelial cells, secreted into the ducts, and excreted into the seminal fluid.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • Cellular localization

    Cell membrane and Secreted > extracellular space. Found in the seminal fluid. Secreted after cleavage of its C-terminus.
  • Information by UniProt
  • Alternative names

    • 2410039E18Rik
    • AI313909
    • C79772
    • CAP1
    • Channel Activating Protease 1
    • mCAP1
    • Prostasin
    • Prostasin heavy chain
    • protease, serine, 8
    • Prss8
    • Serine protease 8
    see all
  • Database links


Our Abpromise guarantee covers the use of ab213884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Mouse Prostasin ELISA Kit (ab213884) Standard Curve.



ab213884 has not yet been referenced specifically in any publications.

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