Overview

  • Product name
    Mouse Prothrombin ELISA Kit
    See all Prothrombin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    1 20 2.48ng/ml 0.148 5.96%
    2 20 11.9ng/ml 0.935 7.86%
    3 20 41.8ng/ml 3.83 9.16%
    Inter-assay
    Sample n Mean SD CV%
    1 10 2.24ng/ml 0.159 7.11%
    2 10 12.9ng/ml 1.27 9.85%
    3 10 47.2ng/ml 2.68 5.68%
  • Sample type
    Serum, Plasma, Tissue Extracts, Cell culture media
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.386 ng/ml
  • Range
    1 ng/ml - 500 ng/ml
  • Recovery

    105.85 %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Prothrombin ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of prothrombin in Mouse plasma, serum, tissue extracts and cell culture media. The assay will not detect Mouse Thrombin. 

    Mouse Prothrombin will bind to the capture antibody coated on the microtiter plate. Thrombin and thrombin-antithrombin complex will not react with the plate. After appropriate washing steps, biotinylated primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of prothrombin. The amount of color development is directly proportional to the concentration of Prothrombin in the sample.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer 1 x 50ml
    Anti-mouse Prothrombin primary antibody 1 vial
    Anti-prothrombin coated Microtiter Plate (8 x 12 wells) 1 unit
    HRP-conjugated Streptavidin 1 vial
    Prothrombin Standard 1 vial
    TMB substrate solution 1 x 10ml
  • Research areas
  • Function
    Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
  • Tissue specificity
    Expressed by the liver and secreted in plasma.
  • Involvement in disease
    Defects in F2 are the cause of factor II deficiency (FA2D) [MIM:613679]. It is a very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels.
    Genetic variations in F2 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
    Defects in F2 are the cause of thrombophilia due to thrombin defect (THPH1) [MIM:188050]. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation. Note=A common genetic variation in the 3-prime untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increased risk of venous thrombosis.
    Defects in F2 are associated with susceptibility to pregnancy loss, recurrent, type 2 (RPRGL2) [MIM:614390]. A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions.
  • Sequence similarities
    Belongs to the peptidase S1 family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
    N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major).
  • Cellular localization
    Secreted > extracellular space.
  • Information by UniProt
  • Alternative names
    • Coagulation factor II
    • F2
    • PT
    • RPRGL2
    • THPH1
    • THRB_HUMAN
    • Thrombin heavy chain
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab157526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

Protocols

References

This product has been referenced in:
  • Ezquer F  et al. Administration of multipotent mesenchymal stromal cells restores liver regeneration and improves liver function in obese mice with hepatic steatosis after partial hepatectomy. Stem Cell Res Ther 8:20 (2017). Mouse . Read more (PubMed: 28129776) »
See 1 Publication for this product

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