Overview

  • Product name

    Mouse PTX3 ELISA Kit
    See all Pentraxin 3/PTX3 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    serum 8 5%
    Inter-assay
    Sample n Mean SD CV%
    serum 3 10.4%
  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    22.3 pg/ml
  • Range

    156.25 pg/ml - 10000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 102 100% - 105%
    Serum 103 95% - 111%
    Hep Plasma 106 105% - 108%
    EDTA Plasma 113 108% - 120%
    Cit plasma 108 106% - 110%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Cow
  • Product overview

    PTX3 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PTX3 protein in mouse serum, plasma, and cell culture supernatant.


     The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    PTX3 (also known as Pentraxin 3 Pentraxin-related protein or TSG-14) is a secreted protein with a role in innate immune response. It is secreted by many cell types during infectious situations. PTX3 plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. Mouse PTX3 shares 81.9 % and 94.2 % sequence homology with human and rat, respectively.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab245713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard Curve comparison between mouse PTX3 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
  • The PTX3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
  • The concentrations of PTX3 were measured in duplicates, interpolated from the PTX3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 12.5%, plasma (EDTA) 12.5%, plasma (heparin) 12.5% and 3T3-L1 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PTX3 concentration was determined to be 36.40 ng/mL in serum, 33.63 ng/mL in plasma (citrate), 50.51 ng/mL in plasma (EDTA), 51.03 ng/mL in plasma (heparin), and 8.37 ng/mL in 3T3-L1 cell culture supernatant.

Protocols

References

ab245713 has not yet been referenced specifically in any publications.

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