Overview

  • Product name
    Mouse RANKL ELISA Kit (TNFSF11)
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 4 pg/ml
  • Range
    2.74 pg/ml - 2000 pg/ml
  • Recovery

    96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 106.5 95% - 115%
    Serum 95.05 83% - 104%
    Plasma 89.2 74% - 104%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human, Pig
  • Product overview

    Abcam’s RANKL Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse RANKL in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse RANKL coated on a 96- well plate. Standards and samples are pipetted into the wells and RANKL present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse RANKL antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RANKL bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Mouse RANKL (lyophilized) 2 vials
    RANKL Microplate (12 strips x 8 wells) 1 unit
    Recombinant Mouse RANKL Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
  • Tissue specificity
    Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
  • Involvement in disease
    Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development.
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.
  • Cellular localization
    Cytoplasm; Secreted and Cell membrane.
  • Information by UniProt
  • Alternative names
    • CD254
    • CD254 antigen
    • hRANKL2
    • ODF
    • OPGL
    • OPTB2
    • Osteoclast differentiation factor
    • Osteoprotegerin ligand
    • RANKL
    • receptor activator of nuclear factor kappa B ligand
    • Receptor activator of nuclear factor kappa-B ligand
    • sOdf
    • soluble form
    • TNF-related activation-induced cytokine
    • TNF11_HUMAN
    • Tnfsf11
    • TRANCE
    • tumor necrosis factor (ligand) superfamily, member 11
    • Tumor necrosis factor ligand superfamily member 11
    see all
  • Database links
    see all

Applications

Our Abpromise guarantee covers the use of ab100749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • ab100749 RANKL (TNFSF11) Mouse ELISA Kit
    ab100749 RANKL (TNFSF11) Mouse ELISA Kit

    RANKL measured in biological fluids showing quantity (pg) per mL of tested sample

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab100749

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab100749

Protocols

References

This product has been referenced in:
  • Huang G  et al. Platelet-rich plasma shows beneficial effects for patients with knee osteoarthritis by suppressing inflammatory factors. Exp Ther Med 15:3096-3102 (2018). Read more (PubMed: 29599843) »
  • Loveridge CJ  et al. Analysis of Nkx3.1:Cre-driven Erk5 deletion reveals a profound spinal deformity which is linked to increased osteoclast activity. Sci Rep 7:13241 (2017). Read more (PubMed: 29038439) »

See all 2 Publications for this product

Publishing research using ab100749? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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18 reviews or Q&A

10
Votes: Highest First

Phoned to ask if this kit will react with the human RANKL.

Thank you for contacting us. I have had the opportunity to discuss your question with the lab.

This product has to our knowledge not been tested on human serum samples. However the sequence homology between the two species is such that it is...

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We want to use the kit ab100749 with automatic washer, but amount of wash buffer supplied with kit is not sufficient for that. Is it possible to prepare wash buffer in our lab from commercially available chemicals?



The 20X Wash Buffer contained in this kit is available for purchase in our catalogue under reference number https://www.abcam.com/ab173280.html.

Abreview for RANKL Mouse ELISA Kit

 Good Good 4/5 (Ease of Use)
Abreviews

Sample: Mouse Serum (Test with human serum to see specificity)
Specification: Test with human serum to see specificity
Amount of Sample: 25 ul each µg
Type of Kit Used: ELISA
Positive control: Positive controls are mice serum, the RANKL level is 0-200 pg/ml, the human serum I tested with this kit is 140 pg/ml, so this kit can also be used for human samples, not specific for mouse.
Did you use the Abcam Kit protocol?: Yes
Username

Mr. GJ Xia

Verified customer

Submitted Jan 11 2013

Thank you for the reply to Olga, I will take over now. Did you also have OPG ELISA kit to offer?

RANKL (ab100749) seems to be suitable, are you willing to quote in 25 kits? (or 25 plates if you have kits with more than one plate)

I ...

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Thank you for your email.

Yes, we do have OPG kits available. The catalogue number is ab100617 and ab100733, one is specific to human and other one to mouse.

https://www.abcam.com/Osteoprotegerin-Human-ELISA-Kit-1-x-96-Well-Plate-ab10...

Read More

Does Rapidtest provide following Elisa kit? If so then I need informations how to do the ELISA and the price as well.

RANK-L and OPG. 25 pre-coated ELISA kit to measure samples from serum and EDTA plasma samples? Are control included in the ki...

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Thank you for contacting us.

Could you please provide the species you are interested in? We have mouse RANKL ELISA kit to use for serum as well as plasma.

https://www.abcam.com/RANKL-mouse-ELISA-Kit-1-x-96-Well-Plate-ab100749.html
Read More

I'd like to test the RANKL kit in human for you and get the discount code.

I am very pleased to hear you would like to accept our offer and test ab100749 in human. This code will give you: $470 off a future order before the expiration date. To redeem this offer, please submit an Abreview forhuman and include this code in the ...

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Will this kit work with human RANKL? Is it possible to test this?

Thank you very much for your interest in ab100749.

To our knowledge, ab100749 has not been tested in human. Therefore, I can offer a discount off a future purchase if you buy ab100749 now, test it inhuman and submit feedback to us in the form ...

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A while ago we tested the RANKL ELISA kit (ab100749). Our assay did not work. There was no quantifiable levels of RANK in our samples.

We would like to come back to this experiment and therefore would like to troubleshoot with you. We used a ...

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Thank you for contacting Abcam regarding ab100749.


I have confirmed with the laboratory that EDTA will not interfere with this kit.When we tested normal mouse serum and plasma samples with this kit, the measured levels were around 300 pg/...

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According to our phone conversation, attached is my results. I really appreciate your help.
Best Regards,

Thank you for sending along the data.

In both graphs the concentrations are listed in log scale but the ODs are shown in linear scale. The ODs should also be in log scale on the y axis.

The OPG data looks as expected, where the conc...

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Phone call enquiring as to whether the was buffer from the Osteoprotegerin Mouse ELISA Kit RANKL (ab100733) and mouse ELISA Kit (ab100749)

Thank you for calling us on Friday.

I can now confirm to you that the wash buffers of the two kits (ab100733 and ab100749) are interchangeable and if you want you could just make up one for one kit and use with both kits until you need to mak...

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