Overview

  • Product name

    Mouse RANTES ELISA Kit
    See all RANTES kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 8 6.5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 4.2%
  • Sample type

    Cell culture supernatant, Serum
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    5.04 pg/ml
  • Range

    7.81 pg/ml - 500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 103 99% - 105%
    Serum 117 111% - 123%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse, Rat
    Does not react with: Cow, Human
  • Product overview

    RANTES in vitro SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of RANTES protein in mouse serum, platelet poor plasma, and cell culture supernatant.


    The SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    RANTES is a small chemoattractant for monocytes, eosinophils, basophils, and some T cells. RANTES (also known as CCL5) is produced by a variety of cells including endothelium, fibroblasts, respiratory epithelium, smooth muscle, astrocytes, platelets, eosinophils, keratinocytes, and CD4+ and CD8+ T cells in response to cytokine stimulation.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    20X Mouse RANTES Capture Antibody 1 x 330µl
    20X Mouse RANTES Detector Antibody 1 x 330µl
    Antibody Diluent 5BI 1 x 6ml
    Mouse RANTES Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
  • Tissue specificity

    T-cell and macrophage specific.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications

    N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
    The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Beta chemokine RANTES
    • Beta chemokine RANTES precursor
    • C C motif chemokine 5
    • CCL 5
    • CCL5
    • CCL5_HUMAN
    • Chemokine (C C motif) ligand 5
    • Chemokine CC Motif Ligand 5
    • D17S136E
    • EoCP
    • Eosinophil chemotactic cytokine
    • MGC17164
    • RANTES(4-68)
    • Regulated upon activation normally T expressed and presumably secreted
    • SCYA 5
    • SCYA5
    • SIS delta
    • SIS-delta
    • SISd
    • Small inducible cytokine A5
    • Small inducible cytokine A5 (RANTES)
    • Small inducible cytokine subfamily A (Cys Cys) member 5
    • Small-inducible cytokine A5
    • T cell specific protein p288
    • T cell specific protein RANTES
    • T cell specific RANTES protein
    • T cell-specific protein P228
    • T-cell-specific protein RANTES
    • TCP 228
    • TCP228
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab215537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between mouse RANTES SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity with shorter protocol time.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of RANTES were measured in duplicates, interpolated from the RANTES standard curves and corrected for sample dilution. Undiluted samples are as follows: RAW 264.7 + LPS supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RANTES concentration was determined to be 5,081 pg/mL in RAW 264.7 + LPS supernatant.

  • The concentrations of RANTES in rat serum were measured in duplicate, interpolated from the mouse RANTES standard curves and corrected for sample dilution. Undiluted samples are rat serum extract 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).  The mean RANTES concentration was determined to be 3,492 pg/mL in rat serum.

Protocols

References

This product has been referenced in:

See 1 Publication for this product

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