Mouse Relaxin 1 ELISA Kit (ab213885)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 78 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Relaxin 1 ELISA Kit
See all Relaxin 1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 104pg/ml 4.16 = 4% 2 16 612pg/ml 47.12 = 7.7% 3 16 2407pg/ml 154.04 = 6.4% Inter-assay Sample n Mean SD CV% 1 24 108pg/ml 7.71 = 5.2% 2 24 616pg/ml 59.13 = 9.6% 3 24 2227pg/ml 144.75 = 6.5% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
78 pg/ml - 5000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
The Mouse Relaxin 1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213885) is designed for the quantitative measurement of Mouse Relaxin 1 in cell culture supernatants, serum and plasma (heparin or EDTA ).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for Relaxin 1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: R23-R54 (B) & E161-C185 (A)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Relaxin 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Relaxin 1 amount of sample captured in plate.
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Notes
Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to mouse chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix (cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Mouse Relaxin 1 coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Mouse Relaxin 1 antibody 1 x 100µl Lyophilized recombinant Mouse Relaxin 1 standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Function
Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix. -
Tissue specificity
Prostate. Not expressed in placenta, decidua or ovary. -
Sequence similarities
Belongs to the insulin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- H1
- H1RLX
- Preprorelaxin H1
see all -
Database links
- Entrez Gene: 19773 Mouse
- SwissProt: P47932 Mouse
- Unigene: 5033 Mouse
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab213885 has been referenced in 1 publication.
- Pawlicki P et al. Telocytes in the mouse testicular interstitium: implications of G-protein-coupled estrogen receptor (GPER) and estrogen-related receptor (ERR) in the regulation of mouse testicular interstitial cells. Protoplasma 256:393-408 (2019). PubMed: 30187340