Overview

  • Product name

    Mouse Relaxin 1 ELISA Kit
    See all Relaxin 1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Sample 1 16 688pg/ml 45.41 6.6%
    Sample 2 16 1402pg/ml 60.3 4.3%
    Sample 3 16 2366pg/ml 130 5.5%
    Inter-assay
    Sample n Mean SD CV%
    Sample 1 24 651pg/ml 48.2 7.4%
    Sample 2 24 1583pg/ml 95 6%
    Sample 3 24 2425pg/ml 174.6 7.2%
  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    78 pg/ml - 5000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    The Mouse Relaxin 1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213885) is designed for the quantitative measurement of Mouse Relaxin 1 in cell culture supernatants, serum and plasma (heparin or EDTA ).


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for Relaxin 1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: R23-R54 (B) & E161-C185 (A)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Relaxin 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Relaxin 1 amount of sample captured in plate.

  • Notes

    Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to mouse chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix (cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Mouse Relaxin 1 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Mouse Relaxin 1 antibody 1 x 130µl
    Lyophilized recombinant Mouse Relaxin 1 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix.
  • Tissue specificity

    Prostate. Not expressed in placenta, decidua or ovary.
  • Sequence similarities

    Belongs to the insulin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • H1
    • H1RLX
    • Preprorelaxin H1
    • Prorelaxin
    • Prorelaxin H1
    • REL1_HUMAN
    • Relaxin A chain
    • Relaxin H1
    • RLN 1
    • Rln1
    • RLXH1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Mouse Relaxin 1 ELISA Kit (ab213885) Standard Curve.

Protocols

References

ab213885 has not yet been referenced specifically in any publications.

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