Overview

  • Product name

    Mouse Renin 1 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    6 pg/ml
  • Range

    6 pg/ml - 50000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant = 107.3 87% - 132%
    Serum = 130.5 112% - 145%
    Plasma = 134.4 128% - 142%

  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s Renin 1 mouse ELISA kit (ab193728) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Renin 1 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse Renin 1 coated on a 96 well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Renin 1 present in the sample. The wells are washed and biotinylated anti-mouse Renin 1 antibody is added. After washing away unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted into the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color develops in proportion to the amount of Renin 1 bound in each well. Addition of the Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    600X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated mouse Renin 1 detection antibody (lyophilized) 2 vials
    mouse Renin 1 Standards (lyophilized) 2 vials
    Pre-coated mouse Renin1 Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Function

    Functions as a renin and prorenin cellular receptor. May mediate renin-dependent cellular responses by activating ERK1 and ERK2. By increasing the catalytic efficiency of renin in AGT/angiotensinogen conversion to angiotensin I, it may also play a role in the renin-angiotensin system (RAS).
  • Tissue specificity

    Expressed in brain, heart, placenta, liver, kidney and pancreas. Barely detectable in lung and skeletal muscles. In the kidney cortex it is restricted to the mesangium of glomeruli. In the coronary and kidney artery it is expressed in the subendothelium, associated to smooth muscles where it colocalizes with REN. Expressed in vascular structures and by syncytiotrophoblast cells in the mature fetal placenta.
  • Involvement in disease

    Mental retardation, X-linked, with epilepsy
    Parkinsonism with spasticity, X-linked
  • Post-translational
    modifications

    Phosphorylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • ATP6AP2
    • ATP6M8-9
    • ATPase H(+)-transporting lysosomal accessory protein 2
    • ATPase H(+)-transporting lysosomal-interacting protein 2
    • Embryonic liver differentiation factor 10
    • ER-localized type I transmembrane adaptor
    • N14F
    • Renin receptor
    • Renin/prorenin receptor
    • RENR_HUMAN
    • V-ATPase M8.9 subunit
    • Vacuolar ATP synthase membrane sector-associated protein M8-9
    see all

Applications

Our Abpromise guarantee covers the use of ab193728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

Protocols

References

ab193728 has not yet been referenced specifically in any publications.

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