Overview

  • Product name
    Mouse Retinol Binding Protein 4 ELISA Kit
    See all Retinol binding Protein 4 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    overall 5 4.5%
    Inter-assay
    Sample n Mean SD CV%
    overall 3 3.8%
  • Sample type
    Cell culture supernatant, Urine, Serum, Cell culture extracts, Tissue Extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    1.6 pg/ml
  • Range
    12.5 pg/ml - 800 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 105 97% - 112%
    Urine 98 94% - 104%
    Serum 101 96% - 106%
    Heparin Plasma 94 91% - 96%
    EDTA Plasma 99 94% - 103%
    Citrate Plasma 99 92% - 104%
    Tissue Homogenate 112 104% - 130%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Sheep, Rabbit, Goat, Guinea pig, Cow, Dog, Human, Pig
  • Product overview

    Abcam’s Retinol Binding Protein 4 (RBP4) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of RBP4 protein in mouse serum, plasma, urine, cell culture supernatant, cell and tissue extracts.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity: Samples diluted in Sample Diluent NS – 3.3 pg/mL
                         Samples diluted in 1X Cell Extraction Beffer PTR – 1.6 pg/mL

  • Notes

    Retinol-Binding Protein 4 (RBP4) is a small, highly conserved member of the lipocalin superfamily involved in the transportation of retinol (Vitamin A) in blood. Synthesized in hepatocytes and adipocytes, RBP4 is also an adipokine involved in insulin resistance in the AG4KO mouse model. Elevated serum levels of RBP4 are seen in obesity and Type 2 diabetes.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse RBP4 Capture Antibody 1 x 600µl
    10X Mouse RBP4 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BC 1 x 6ml
    Mouse RBP4 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas
  • Function
    Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.
  • Involvement in disease
    Retinal dystrophy, iris coloboma, and comedogenic acne syndrome
  • Sequence similarities
    Belongs to the calycin superfamily. Lipocalin family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • Plasma retinol binding protein
    • Plasma retinol-binding protein
    • Plasma retinol-binding protein(1-176)
    • PRBP
    • PRO2222
    • RBP
    • RBP4
    • RDCCAS
    • RET4_HUMAN
    • Retinol binding protein 4
    • Retinol binding protein 4 interstitial
    • Retinol binding protein 4 plasma
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab202404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • ELISA Protocol Summary
  • Standard curve comparison between mouse RBP4 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep kit shows a 25-fold increase in sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Interpolated values of RBP4 are plotted for the indicated tissue extracts based on an extract load of 30 µg/mL. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 2) are graphed.

Protocols

References

ab202404 has not yet been referenced specifically in any publications.

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