Overview

  • Product name

    Mouse Retinol binding protein 4 ELISA Kit (RBP4)
    See all Retinol binding Protein 4 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.2%
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    6 ng/ml
  • Range

    0.625 ng/ml - 20 ng/ml
  • Recovery

    98 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s Retinol binding protein 4 (RBP4) Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of RBP4 concentrations in mouse plasma, serum, urine and cell culture supernatants.


    An RBP4 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an RBP4 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of RBP4 captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Mouse RBP4 Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Mouse RBP4 Microplate (12 x 8 well strips) 1 unit
    Mouse RBP4 Standard (Lyophilised) 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.
  • Involvement in disease

    Retinal dystrophy, iris coloboma, and comedogenic acne syndrome
  • Sequence similarities

    Belongs to the calycin superfamily. Lipocalin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Plasma retinol binding protein
    • Plasma retinol-binding protein
    • Plasma retinol-binding protein(1-176)
    • PRBP
    • PRO2222
    • RBP
    • RBP4
    • RDCCAS
    • RET4_HUMAN
    • Retinol binding protein 4
    • Retinol binding protein 4 interstitial
    • Retinol binding protein 4 plasma
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab195459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab195459.

Protocols

References

ab195459 has not yet been referenced specifically in any publications.

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