Product nameMouse Retinol Binding Protein 4 Matched Antibody Pair Kit
See all RBP4 kits
Assay typeELISA set
Range15.62 pg/ml - 1000 pg/ml
Species reactivityReacts with: Mouse
Mouse RBP4 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse RBP4.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab202404. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
We’ve listened to you: due to popular demand, we will now provide our Matched Antibody Pair kits in 5x96 tests and 10x96 tests. The 2x96 tests size will be discontinued on 30th June 2020 unless inventory is depleted beforehand.
To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.
Tested applicationsSuitable for: ELISA, IAmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 10 x 96 tests 5 x 96 tests Mouse Retinol Binding Protein 4 Capture Antibody 1 x 100µg 1 x 50µg Mouse Retinol Binding Protein 4 Detector Antibody 1 x 25µg 1 x 12.5µg Mouse Retinol Binding Protein 4 Lyophilized Protein 1 vial 1 vial
FunctionDelivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.
Involvement in diseaseDefects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency) [MIM:180250]. This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium.
Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
- Information by UniProt
Our Abpromise guarantee covers the use of ab214479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IA||Use at an assay dependent concentration.|
ab214479 has not yet been referenced specifically in any publications.