Overview

  • Product name

    Mouse Retinol Binding Protein ELISA Kit (RBP)
    See all Retinol binding protein kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type

    Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.152 ng/ml
  • Range

    0.625 ng/ml - 20 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s Retinol Binding Protein (RBP) Mouse ELISA kit (ab190535) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Retinol Binding Protein (RBP) in mouse serum and plasma.

    In this assay the Retinol Binding Protein (RBP) present in samples reacts with the anti-Retinol Binding Protein (RBP) antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-RBP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound RBP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of RBP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of RBP in the test sample. The quantity of RBP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab190535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab190535 Retinol Binding Protein (RBP) Mouse ELISA Kit.

Protocols

References

ab190535 has not yet been referenced specifically in any publications.

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