Overview

  • Product name

    Mouse SAA ELISA Kit
    See all Serum Amyloid A kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 5.5%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 8.3%
  • Sample type

    Serum, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    39 pg/ml
  • Range

    0.22 ng/ml - 2.5 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 122 119% - 125%
    EDTA Plasma 113 111% - 114%
    Cit plasma 127 124% - 129%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Cow
  • Product overview

    SAA in vitro SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse SAA1 protein in serum, plasma (citrate), and plasma (EDTA).


    The SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


     

  • Notes

    Mouse SAA1 (serum amyloid A-1 protein) is a secreted 102 amino acid, acute phase protein encoded by the Saa1 gene. The SAA proteins are a family of apolipoproteins that are associated with high-density lipoprotein (HDL). Four SAA isoforms have been currently characterized in mice; SAA1 shares 91%, 70%, and 54% sequence identify with mouse SAA2, SAA3, and SAA4, respectively. SAA1 and SAA2 are expressed primarily by the liver and are regulated by the cytokines IL-1, IL-6 and TNF-α, and the levels can increase as much as 1000 fold during inflammation. SAA1 is also associated with certain cancers, atherosclerosis, and rheumatoid arthritis. SAA1 is a precursor of amyloid A, fibril deposits of which may result in inflammatory amyloidosis.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPR 1 x 6ml
    10X Mouse SAA Capture Antibody 1 x 600µl
    10X Mouse SAA Detector Antibody 1 x 600µl
    Mouse SAA Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Relevance

    Function: Major acute phase reactant. Apolipoprotein of the HDL complex. Tissue specificity: Expressed by the liver; secreted in plasma. Disease: Note=Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Note=Elevated serum SAA protein levels may be associated with lung cancer. Similarity: Belongs to the SAA family. PTM: This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.
  • Alternative names

    • Amyloid fibrils
    • SAA 2
    • Serum amyloid A1 isoform 2
    • TP53I4
    • Amyloid fibril protein AA
    • Amyloid protein A
    • Fibrils
    • MGC111216
    • OC
    • OTTMUSP0000004557
    • PIG 4
    • PIG4
    • SAA
    • SAA 1
    • SAA_HUMAN
    • SAA1
    • SAA2
    • serum amyloid A
    • Serum amyloid A 1
    • Serum amyloid A protein precursor
    • Serum amyloid A1 isoform 1
    • Serum amyloid protein A(4-101)
    • Tumor protein p53 inducible protein 4
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab215090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of serum amyloid A were measured in duplicate, interpolated from the serum amyloid A standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:6000, plasma (citrate) 1:3750, and plasma (EDTA) 1:3750. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean serum amyloid A concentration was determined to be 23.7 µg/mL in serum, 11.5 µg/mL in plasma (citrate) and 11.8 µg/mL in plasma (EDTA).

Protocols

References

ab215090 has not yet been referenced specifically in any publications.

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