Overview

  • Product name
    Mouse SAA ELISA Kit (SAA1)
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type
    Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    10.7 ng/ml
  • Range
    31.25 ng/ml - 2000 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Mouse SAA ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Serum Amyloid A in mouse samples.


    In this assay the SAA present in samples reacts with the anti-SAA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-SAA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Serum Amyloid A. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Serum Amyloid A in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X HRP-conjugated anti-mouse SAA antibody 1 x 150µl
    20X Wash Buffer Concentrate 1 x 50ml
    5X Diluent Concentrate 1 x 50ml
    Chromogen Substrate Solution 1 x 12ml
    Mouse SAA Calibrator (lyophilized) 1 vial
    Mouse SAA ELISA Microplate 1 unit
    Stop Solution 1 x 12ml
  • Research areas
  • Relevance
    The serum amyloid A (SAA) family comprises a number of differentially expressed lipoproteins, acute phase SAA1 and SAA2, the former being a major component in plasma, and constitutive SAA's (C-SAAs). Although the liver is the primary site of synthesis of both SAA types, extrahepatic production has been reported. The in vivo concentrations increase by as much as 1000 fold during inflammation. Several studies have expressed it's importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations. SAA rises earlier and more sharply than CRP. SAA enhances the binding of HDL's to macrophages and thus helps the delivery of lipid to sites of injury for use in tissue repair. It is thus thought to be an integral part of the disease process. In addition, recent experiments suggest that SAA may play a "houekeeping" role in normal human tissues. Elevated levels of SAA over time predispose secondary amyloidosis, extracellular accumulation of amyloid fibrils, derived from a circulating precursor, in various tissues and organs. The most common form of amyloidosis occurs secondary to chronic inflammatory disease, particularly rheumatoid artheritis.
  • Cellular localization
    Secreted
  • Alternative names
    • Amyloid fibril protein AA
    • Amyloid protein A
    • MGC111216
    • OTTMUSP00000045357
    • PIG4
    • SAA
    • SAA1
    • SAA2
    • Serum amyloid A 1
    • Serum amyloid A 1 protein
    • serum amyloid A protein
    • Serum amyloid A protein precursor
    • Serum amyloid A1 isoform 1
    • Serum amyloid A1 isoform 2
    • TP53I4
    • Tumor protein p53 inducible protein 4
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab157723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SAA measured in various biological samples showing quantity (ng) per mL of sample tested. For mouse samples (Ms) dilutions of 1:1000 were used.

  • Representative standard curve using ab157723 SAA Mouse ELISA Kit.

Protocols

References

This product has been referenced in:
  • Cavalera M  et al. Dietary rose hip exerts antiatherosclerotic effects and increases nitric oxide-mediated dilation in ApoE-null mice. J Nutr Biochem 44:52-59 (2017). Functional Studies ; Mouse . Read more (PubMed: 28399420) »
  • Wang J  et al. Myeloid Cell-Specific Lipin-1 Deficiency Stimulates Endocrine Adiponectin-FGF15 Axis and Ameliorates Ethanol-Induced Liver Injury in Mice. Sci Rep 6:34117 (2016). Sandwich ELISA ; Mouse . Read more (PubMed: 27666676) »
See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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