Overview

  • Product name

    Mouse SAP ELISA Kit
    See all SAP kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Mouse serum 5 2.4%
    Inter-assay
    Sample n Mean SD CV%
    Mouse serum 3 5.7%
  • Sample type

    Cell culture supernatant, Serum, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    11.3 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 118 109% - 124%
    Cell culture media 98 95% - 102%
    EDTA Plasma 109 95% - 122%
    Citrate Plasma 97 83% - 109%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rat, Cow, Human
  • Product overview

    SAP in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of SAP protein in mouse serum, plasma, and cell culture supernatants.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution 1 x 1ml
    Antibody Diluent 5BR 1 x 6ml
    Denaturant 1 x 500µl
    10X Mouse SAP Capture Antibody 1 x 600µl
    10X Mouse SAP Detector Antibody 1 x 600µl
    Mouse SAP Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

Associated products

Applications

Our Abpromise guarantee covers the use of ab235639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between Mouse SAP SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • The SAP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of SAP were measured in duplicates, interpolated from the SAP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:100,000, plasma (citrate) 1:100,000, plasma (EDTA) 1:100,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SAP concentration was determined to be 197 µg/mL in neat serum, 98.7 µg/mL in neat plasma (citrate) and 176 µg/mL in neat plasma (EDTA).

  • The concentrations of SAP were measured in duplicates, interpolated from the SAP standard curves and corrected for sample dilution. Undiluted samples are as follows: mouse kidney tissue supernatant 1:100 and mouse lung tissue supernatant 1:100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SAP concentration was determined to be 43 ng/mL in neat kidney tissue supernatant and 113 ng/mL in neat lung tissue supernatant.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SAP concentration was determined to be 193 µg/mL in neat CD-1 serum, 433 µg/mL in neat C57BL-6 serum, and 583 µg/mL in neat BALB/c serum.

Protocols

References

ab235639 has not yet been referenced specifically in any publications.

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