Overview

  • Product name

    Mouse SAP ELISA Kit (PTX2)
    See all Serum Amyloid P/SAP kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Sample 1 16 5.68ng/ml 0.33 5.8%
    Sample 2 16 34ng/ml 1.43 4.2%
    Sample 3 16 61ng/ml 3.05 5%
    Inter-assay
    Sample n Mean SD CV%
    Sample 1 24 5.2ng/ml 0.39 7.5%
    Sample 2 24 39ng/ml 2.11 5.4%
    Sample 3 24 74ng/ml 4.59 6.2%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 50 pg/ml
  • Range

    1.56 ng/ml - 100 ng/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    The Mouse SAP Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213888) is designed for the quantitative measurement of Mouse SAP in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for SAP has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: Q21- D224) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SAP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Serum Amyloid P / SAP amount of sample captured in plate.

  • Notes

    SAP also known as PTX2 or APCS, is the identical serum form of amyloid P component (AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterized by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Mouse SAP coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Mouse SAP antibody 1 x 130µl
    Lyophilized recombinant Mouse SAP standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
  • Tissue specificity

    Found in serum and urine.
  • Involvement in disease

    Note=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits.
  • Sequence similarities

    Belongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • Post-translational
    modifications

    N-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148).
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • 9.5S alpha 1 glycoprotein
    • 9.5S alpha-1-glycoprotein
    • Amyloid P component serum
    • APCS
    • MGC88159
    • Pentaxin related
    • Pentraxin 2
    • PTX 2
    • PTX2
    • SAMP_HUMAN
    • SAP
    • Serum Amyloid P
    • Serum amyloid P component
    • Serum amyloid P-component(1-203)
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Mouse Serum Amyloid P / SAP ELISA Kit (ab213888) Standard Curve.

Protocols

References

ab213888 has not yet been referenced specifically in any publications.

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