Mouse SCF ELISA Kit (ab100740)

Thank you for your inquiry.

Unfortunately, we do not have any cross reactivity data for testing rat samples using the mouse stem cell factor ELISA kit (ab100740). Based on online literature though, mouse SCF has ˜93% homology with rat SCF so there is certainly a good chance of getting cross reactivity. However, the only way to know for sure would be to test. If it helps, both the capture and detection antibody in ab100740 are polyclonal goat IgG. While ab100740 is neither designed nor validated with tissue lysate samples, our kits are very accommodating to many different samples types so lysates will most likely work. We recommend diluting the samples at least 5-fold with Assay Diluent B (Item E) to minimize any effects of the detergents in the lysis buffer. The samples may need to be diluted further but this would need to be determined by the the end-use empirically.

Basically, a lysis buffer must meet the following specifications:

A) has relatively low salt content (700 mM or less)
B) does not contain sodium azide
C) does not contain more than 0.1% SDS
D) does not contain more than 10 mM reducing agents (beta-mercaptoethanol or dithiothreitol).

This would include any buffers used for immunoprecipitations, including RIPA buffer.

I hope this information is helpful. Please do not hesitate to contact us again with any further

Thank you for your call today and for letting us know about the trouble with this kit.
As we discussed, I'm sending a free of charge kit which should arrive around Tuesday of next week on the order ***. I have requested that the components are checked for precipitation and shortages.
I apologize again for the problems with this latest kit. Please let me know how this replacement kit works, and let me know if you have any questions or if there is anything else that we can do for you.

Thank you for contacting us.

First, I want to apologize to you for the delay in gathering this information. I hope this did not cause you any inconveniences.

In regards to using rat tissues in our SCF Mouse ELISA kit, there is no cross reactivity data testing this mouse kit (ab100740) with rat samples so we do not know. However, we do know that the antibody pair is binding somewhere on the extracellular domain of the SCF protein and that mouse and rat share ˜93% homology on the extracellular domain. Therefore there is a good chance that there will be cross reactivity. Nevertheless, the only way to be certain would be to test.



As to your questions about using urine with ab100786 and ab119602.

Ab119602 will work with urine future datasheets will include this.

While ab100786 was not validated with urine samples (only serum, plasma, and cell culture supernatants) these types of kit are very accommodating to many types of samples so I see no reason why urine or any other body fluid for that matter would not be compatible with this kit.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.