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Can I use this kit with rat tissues?
Asked on Apr 02 2012
Thank you for your inquiry.
Unfortunately, we do not have any cross reactivity data for testing rat samples using the mouse stem cell factor ELISA kit (ab100740). Based on online literature though, mouse SCF has ˜93% homology with rat SCF so there is certainly a good chance of getting cross reactivity. However, the only way to know for sure would be to test. If it helps, both the capture and detection antibody in ab100740 are polyclonal goat IgG. While ab100740 is neither designed nor validated with tissue lysate samples, our kits are very accommodating to many different samples types so lysates will most likely work. We recommend diluting the samples at least 5-fold with Assay Diluent B (Item E) to minimize any effects of the detergents in the lysis buffer. The samples may need to be diluted further but this would need to be determined by the the end-use empirically.
Basically, a lysis buffer must meet the following specifications:
A) has relatively low salt content (700 mM or less)
B) does not contain sodium azide
C) does not contain more than 0.1% SDS
D) does not contain more than 10 mM reducing agents (beta-mercaptoethanol or dithiothreitol).
This would include any buffers used for immunoprecipitations, including RIPA buffer.
I hope this information is helpful. Please do not hesitate to contact us again with any further
Answered on Apr 02 2012