• Product name
    Mouse SDF1 ELISA Kit (CXCL12)
    See all SDF1 alpha kits
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 3 pg/ml
  • Range
    9.38 pg/ml - 600 pg/ml
  • Recovery

    99 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 99.34 89% - 107%
    Serum 99 91% - 107%
    Plasma 100.36 90% - 107%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Mouse SDF1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab100741) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse SDF1 in serum, plasma and cell culture supernatants. (Mouse SDF1 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

    This assay employs an antibody specific for mouse SDF1 coated on a 96-well plate. Standards and samples are pipetted into the wells and SDF1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse SDF1antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SDF1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Mouse SDF1 (lyophilized) 2 vials
    Recombinant Mouse SDF1 Standard (lyophilized) 2 vials
    SDF1 Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1. SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry.
  • Alternative names
    • C-X-C motif chemokine 12
    • Chemokine (C X C motif) ligand 12
    • CXCL12
    • hIRH
    • Intercrine reduced in hepatomas
    • IRH
    • PBSF
    • Pre B cell growth stimulating factor
    • SCYB12
    • SDF 1
    • SDF 1 beta
    • SDF 1b
    • SDF1
    • SDF1 alpha (3-67)
    • SDF1 beta (3-72)
    • SDF1A
    • SDF1B
    • Stromal cell derived factor 1
    • TLSF a
    • TLSF b
    • TLSFa
    • TLSFb
    • TPAR1
    see all


Our Abpromise guarantee covers the use of ab100741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • SDF1 measured in culture supernatants from control or activated cells (P/L), stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) with the addition of 1 ug x mL-1 of LPS (Sigma) for the last 6 hours, and in biological fluids (duplicates +/- SD). All samples tested undiluted.

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Representative Standard Curve using ab100741

  • Representative Standard Curve using ab100741



This product has been referenced in:
  • Li X  et al. Transplantation of skin mesenchymal stem cells attenuated AngII-induced hypertension and vascular injury. Biochem Biophys Res Commun 497:1068-1075 (2018). Read more (PubMed: 29481801) »
  • Shi W  et al. Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury. Acta Biomater 45:247-261 (2016). Read more (PubMed: 27592818) »

See all 3 Publications for this product

Customer reviews and Q&As


Good Excellent 5/5 (Ease of Use)
I used the Mouse SDF-1 ELISA kit to check the levels in mouse retinal tissues and it worked.

Dr. Finny Monickaraj

Verified customer

Submitted Aug 19 2015

Both antibodies used in this kit recognize a region within Lys22­ - Lys89 (Accession # Q6ICW0). Thus, although not previously tested, we do not expect an additional sequenced fused to the C-terminus to interfere with detection.

Thank you for your reply.

You may want to try the new lots and take the precautions listed in the previous email. The lab does not think that anything is wrong with the standards as all lots are quality control tested. Just take the necessary...

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Thank you for your inquiry and your patience while I looked into this further.

The lab has QC'ed these standards for the kit and the certificate of analysis for a recent batch is attached.

The150 rpm is a little fast but it is not l...

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Thank you for your reply.

Just to clarify, the diluted/working standards (600, 200, 66.7, 22.2, 7.4, 2.5, 0.82 pg/ml) should not be stored. The reconstituted standard (50 ng/ml) can be stored at -80ºC for 1-2 weeks at most. Remember, an ...

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Thank you very much for your email.

I am sorry to hear about the customer who has decided to pull out from getting this product. We have helped your customer as far as we can by providing them credit note which you have used for order 112737. ...

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Thank you for your inquiry.

No, the protocol is right. Steps 4 and 5 are not the same as steps 6 and 7. Steps 4 and 5 involve the biotinylated antibody and steps 6 and 7 involved the streptavidin-HRP solution.

Yes, step 6 in "A...

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Thank you for your inquiry.

We know of the IL6 ELISA kit being tested in liver tissue lysate, but unfortunately we do not have any info on brain lysate.

For the SDF1 alpha ELISA kit, we haven't tested tissues with this kit but it wou...

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After reviewing the standard OD data, it doesn’t look like there is any significant detection or linear pattern. Did you run any samples as this would help determine if the issue is just with the standard protein or a common detection component? Stri...

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Thank you for contacting us. I am happy to offer you some tips to help you improve better results from this kit. I would also appreciate if you could send me the raw OD data, so we can try to find out why the standards provided such low values. ...

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