Overview

  • Product name
    Anti-Mouse Serum Albumin antibody (HRP)
    See all Mouse Serum Albumin primary antibodies
  • Description
    Goat polyclonal to Mouse Serum Albumin (HRP)
  • Host species
    Goat
  • Conjugation
    HRP
  • Tested applications
    Suitable for: ELISA, WB, ICC, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Full length protein (Mouse).

  • Positive control
    • Liver (Human and mouse)

Properties

Applications

Our Abpromise guarantee covers the use of ab19195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000 - 1/100000.
WB 1/1000 - 1/30000. Predicted molecular weight: 69 kDa.
ICC 1/200 - 1/500.
IHC-P Use at an assay dependent dilution.

Target

  • Function
    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificity
    Plasma.
  • Sequence similarities
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alb 1 antibody
    • alb antibody
    • ALBU_MOUSE antibody
    • Albumin 1 antibody
    • Albumin antibody
    • Serum albumin antibody
    see all

Images

  • All lanes : Anti-Mouse Serum Albumin antibody (HRP) (ab19195) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Mouse) Tissue Lysate (ab7935)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 69 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds

References

This product has been referenced in:
  • Grevys A  et al. A human endothelial cell-based recycling assay for screening of FcRn targeted molecules. Nat Commun 9:621 (2018). Read more (PubMed: 29434196) »
  • Swiercz R  et al. Loss of expression of the recycling receptor, FcRn, promotes tumor cell growth by increasing albumin consumption. Oncotarget 8:3528-3541 (2017). Read more (PubMed: 27974681) »
See all 5 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us.
As we haven't tested the cross-reactivity for this antibodies, it is very much likely that they will react with other albumins.
Therefore, I would suggest to use milk as a blocking agent (0.5% Skim milk/PBS for 1 hour). However, I would suggest not to use Biotin based detection method to avoid high background.
In addition, as anti-goat antibodies can cross-react with IgG from cows, I would actually rather suggest ab24325 or ab34807 for your customer.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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https://www.abcam.com/abreviews

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Answer

Thank you for contacting us. Serum albumin may be a suitable loading control, but I am not sure if levels will vary from one animal to another, for example healthy vs. diseased. These three antibodies detect mouse serum albumin: ab19194 goat polyclonal ab19195 (same as ab19194 but conjugated to HRP) ab85786 rabbit polyclonal I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us. Feel free to call us any time between 8am and 8pm EST at 888-77-ABCAM. I am not sure what you mean by detecting the antibody on a nanofiber film. Is this film a matrix for growing cells? If so, you could use an ICC staining protocol such as the one linked below: https://www.abcam.com/index.html?pageconfig=resource&rid=11417 Alternatively, is the nanofiber film similar to a WB membrane? In that case, you could use a Dot Blot protocol: https://www.abcam.com/index.html?pageconfig=resource&rid=11452 I hope this helps, please let me know if you need any additional information.

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