Mouse soluble IL-2 Receptor Antibody Pair - BSA and Azide free (ab253497)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse
Overview
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Product name
Mouse soluble IL-2 Receptor Antibody Pair - BSA and Azide free -
Assay type
ELISA set -
Range
46.875 pg/ml - 3000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Both capture and detector antibodies are recombinant rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
The Antibody Pair can be used to quantify Mouse soluble IL-2 Receptor. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab277724), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Mouse soluble IL-2 Receptor Capture Antibody (unconjugated) 1 x 100µg Mouse soluble IL-2 Receptor Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Receptor for interleukin-2. -
Involvement in disease
Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
Sequence similarities
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD25
- IL-2 receptor subunit alpha
- IL-2-RA
see all -
Database links
- Entrez Gene: 16184 Mouse
- SwissProt: Q8C2H9 Mouse
- SwissProt: P01590 Mouse
- Unigene: 915 Mouse
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab253497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Example of Mouse soluble IL-2 Receptor standard curve.
Representative standard curve from corresponding SimpleStep ELISA® Kit (ab277724), which uses the same antibody pair. Note that the reference range provided for the pair is an estimation based on the performance of the related kit.
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Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253497)To learn more about the advantages of recombinant antibodies see here.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab253497 has not yet been referenced specifically in any publications.
