Key features and details
- Sensitivity: 5 pg/ml
- Range: 5 pg/ml - 1200 pg/ml
- Sample type: Cell Lysate, Tissue Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse Sonic Hedgehog-N ELISA Kit
See all Sonic Hedgehog N kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell Lysate, Tissue Lysate
Assay typeSandwich (quantitative)
Sensitivity< 5 pg/ml
Range5 pg/ml - 1200 pg/ml
Sample specific recovery Sample type Average % Range Cell Lysate 126.2 114% - 137% Tissue Lysate 130.3 114% - 150%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Abcam’s Shh-N Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse Shh-N in cell lysates and tissue lysates.
This assay employs an antibody specific for mouse Shh-N coated on a 96-well plate. Standards and samples are pipetted into the wells and Shh-N present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Shh-N antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Shh-N bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 2X Cell Lysis Buffer 1 x 5ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml 5X Sample Diluent Buffer 1 x 10ml Biotinylated anti-mouse Shh-N 2 vials Recombinant Mouse Shh-N Standard (lyophilized) 2 vials Shh-N Microplate (12 x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
ab155450 has not yet been referenced specifically in any publications.