Key features and details
- Sensitivity: 5 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse Sonic Hedgehog-N ELISA Kit
See all Sonic Hedgehog N kits
Intra-assay Sample n Mean SD CV% overall < 10% Inter-assay Sample n Mean SD CV% overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 5 pg/ml
Range4.1 pg/ml - 1000 pg/ml
Sample specific recovery Sample type Average % Range Serum 78.88 71% - 87% Plasma 77.03 71% - 79% Cell culture media 82.11 78% - 86%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Abcam’s Shh-N Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Mouse Shh-N in serum, plasma (using EDTA and Citrate as an anticoagulant, heparin is not recommended), and cell culture supernatants.
This assay employs an antibody specific for Mouse Shh-N coated on a 96-well plate. Standards and samples are pipetted into the wells and Shh-N present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Shh-N antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Shh-N bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-mouse Shh-N 2 vials Recombinant Mouse Shh-N Standard (lyophilized) 2 vials Shh-N Microplate (12 x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
ab155451 has not yet been referenced specifically in any publications.