Overview

  • Product name

    Mouse Specific HRP/AEC (ABC) Detection IHC kit
    See all HRP/AEC Detection Kit kits
  • Product overview

    Mouse specific HRP/AEC (ABC) IHC Detection Kit ab64258 is a complete kit to detect a primary antibody raised in mouse, using HRP-labeled-streptavidin and a biotinylated anti-mouse secondary antibody.


    It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and AEC substrate.


    In the method:
    - a sample is incubated with an unconjugated primary antibody specific to the target antigen
    - a biotinylated secondary antibody then binds to the primary antibody
    - HRP-labeled streptavidin then binds to the secondary antibody
    - the HRP produces a colored substance at the site of primary antibody binding by reacting with AEC.

  • Notes

    For alternative AEC streptavidin-biotin IHC detection kits see:
    - anti-Mouse / anti-Rabbit HRP-AEC IHC kit ab93705
    - Anti-Rabbit HRP-AEC IHC Kit ab64260

    For high sensitivity AEC HRP micro-polymer IHC detection kits see:
    - Anti-Mouse / anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236467
    - Anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236468

    Alternatively, see HRP-DAB IHC detection kits.

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Tested applications

    Suitable for: IHC-Pmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 15 ml
    AEC Single Solution 1 x 15ml
    Biotinylated goat anti-mouse IgG(H+L) (ab64255) 1 x 15ml
    Hydrogen Peroxide Block 1 x 15ml
    Protein block 1 x 15ml
    Streptavidin Peroxidase 1 x 15ml
  • Research areas

  • Relevance

    An HRP/AEC Detection Kit is a labeled immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. AEC is 3-amino-9-ethyl-carbazole, a chromogenic substrate.
  • Alternative names

    • carbazole
    • Horseradish Peroxidase

Applications

Our Abpromise guarantee covers the use of ab64258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

Images

  • Immunohistochemical analysis of Bcl-2 expression in Human tonsil, using ab64258 Detection Kit.

References

ab64258 has not yet been referenced specifically in any publications.

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