Mouse Specific HRP/AEC (ABC) Detection IHC kit (ab64258)

Submit a review Submit a question
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mouse/HRP/AEC Detection Kit (ab64258)

    Overview

    • Product name

      Mouse Specific HRP/AEC (ABC) Detection IHC kit
      See all HRP/AEC Detection Kit kits

    • Product overview

      Mouse specific HRP/AEC (ABC) IHC Detection Kit ab64258 is a complete kit to detect a primary antibody raised in mouse, using HRP-labeled-streptavidin and a biotinylated anti-mouse secondary antibody.


      It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and AEC substrate.


      In the method:
      - a sample is incubated with an unconjugated primary antibody specific to the target antigen
      - a biotinylated secondary antibody then binds to the primary antibody
      - HRP-labeled streptavidin then binds to the secondary antibody
      - the HRP produces a colored substance at the site of primary antibody binding by reacting with AEC.

    • Notes

      For alternative AEC streptavidin-biotin IHC detection kits see:
      - anti-Mouse / anti-Rabbit HRP-AEC IHC kit ab93705
      - Anti-Rabbit HRP-AEC IHC Kit ab64260

      For high sensitivity AEC HRP micro-polymer IHC detection kits see:
      - Anti-Mouse / anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236467
      - Anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236468

      Alternatively, see HRP-DAB IHC detection kits.

      Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

    • Tested applications

      Suitable for: IHC-Pmore details

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 15 ml 15 ml
      AEC Single Solution 1 x 15ml 1 x 15ml
      Biotinylated goat anti-mouse IgG(H+L) (ab64255) 1 x 15ml 1 x 15ml
      Hydrogen Peroxide Block 1 x 15ml 1 x 15ml
      Protein block 1 x 15ml 1 x 15ml
      Streptavidin Peroxidase 1 x 15ml 1 x 15ml

    • Research areas

    • Relevance

      An HRP/AEC Detection Kit is a labeled immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. AEC is 3-amino-9-ethyl-carbazole, a chromogenic substrate.

    • Alternative names

      • carbazole
      • Horseradish Peroxidase

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab64258 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use at an assay dependent dilution.
    Notes
    IHC-P
    Use at an assay dependent dilution.

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mouse/HRP/AEC Detection Kit (ab64258)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mouse/HRP/AEC Detection Kit (ab64258)
      Immunohistochemical analysis of Bcl-2 expression in Human tonsil, using ab64258 Detection Kit.

    References (0)

    Publishing research using ab64258? Please let us know so that we can cite the reference in this datasheet.

    ab64258 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab64258.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com