Mouse Specific HRP/AEC (ABC) Detection IHC kit (ab64258)
Overview
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Product name
Mouse Specific HRP/AEC (ABC) Detection IHC kit
See all HRP/AEC Detection Kit kits -
Product overview
Mouse specific HRP/AEC (ABC) IHC Detection Kit ab64258 is a complete kit to detect a primary antibody raised in mouse, using HRP-labeled-streptavidin and a biotinylated anti-mouse secondary antibody.
It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and AEC substrate.
In the method:
- a sample is incubated with an unconjugated primary antibody specific to the target antigen
- a biotinylated secondary antibody then binds to the primary antibody
- HRP-labeled streptavidin then binds to the secondary antibody
- the HRP produces a colored substance at the site of primary antibody binding by reacting with AEC. -
Notes
For alternative AEC streptavidin-biotin IHC detection kits see:
- anti-Mouse / anti-Rabbit HRP-AEC IHC kit ab93705
- Anti-Rabbit HRP-AEC IHC Kit ab64260For high sensitivity AEC HRP micro-polymer IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236467
- Anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236468
Alternatively, see HRP-DAB IHC detection kits.Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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Tested applications
Suitable for: IHC-Pmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 15 ml AEC Single Solution 1 x 15ml Biotinylated goat anti-mouse IgG(H+L) (ab64255) 1 x 15ml Hydrogen Peroxide Block 1 x 15ml Protein block 1 x 15ml Streptavidin Peroxidase 1 x 15ml -
Research areas
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Relevance
An HRP/AEC Detection Kit is a labeled immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. AEC is 3-amino-9-ethyl-carbazole, a chromogenic substrate. -
Alternative names
- carbazole
- Horseradish Peroxidase
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent dilution.
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| Notes |
|---|
|
IHC-P
Use at an assay dependent dilution. |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mouse/HRP/AEC Detection Kit (ab64258)Immunohistochemical analysis of Bcl-2 expression in Human tonsil, using ab64258 Detection Kit.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab64258 has not yet been referenced specifically in any publications.