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  1. Link

    mouse-specific-hrpdab-abc-detection-ihc-kit-ab64259.pdf

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Mouse specific HRP/DAB (ABC) Detection IHC Kit (ab64259)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (2)Q&A (17)References (33)

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Immunohistochemistry - ab64259
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti Mouse HRP/DAB Detection Kit (ab64259)

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Overview

  • Product name

    Mouse specific HRP/DAB (ABC) Detection IHC Kit
    See all HRP/DAB detection kit kits
  • Product overview

    Mouse specific HRP/DAB (ABC) IHC Detection Kit ab64259 is a complete kit to detect a primary antibody raised in mouse, using HRP-labeled-streptavidin and a biotinylated anti-mouse secondary antibody.


    It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and DAB substrate.


    In the method:
    - a sample is incubated with an unconjugated primary antibody specific to the target antigen
    - a biotinylated secondary antibody then binds to the primary antibody
    - HRP-labeled streptavidin then binds to the secondary antibody
    - the HRP produces a brown colored substance at the site of primary antibody binding by reacting with DAB.

  • Notes

    For alternative streptavidin-biotin IHC detection kits see:
    - Anti-Mouse / anti-Rabbit HRP-DAB IHC kit ab64264 
    - Anti-Rabbit HRP-DAB IHC Kit ab64261

    For high sensitivity HRP micro-polymer IHC detection kits see:
    - Anti-Mouse / anti-Rabbit HRP/DAB Micro-polymer IHC Kit ab236466
    - Anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236469

    Alternatively, see HRP-AEC IHC detection kits.

    For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Tested applications

    Suitable for: IHC-Pmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 15 ml
    50x DAB Chromogen 1 x 0.5ml
    Biotinylated goat anti-mouse IgG(H+L) (ab64255) 1 x 15ml
    DAB substrate 1 x 15ml
    Hydrogen Peroxide Block 1 x 15ml
    Protein block 1 x 15ml
    Streptavidin Peroxidase 1 x 15ml
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • IHC Kits
    • Kits/ Lysates/ Other
    • Kits
    • IHC tools
    • ABC
  • Relevance

    HRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.

Associated products

  • Related Buffer

    • 20x PBS buffer with Tween 20 (ab64247)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab64259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent dilution.
Notes
IHC-P
Use at an assay dependent dilution.

Images

  • Immunohistochemistry - ab64259
    Immunohistochemistry - ab64259Jovicic N et al., PLoS One 10(7). Fig 6D & E. DOI: 10.1371/journal.pone.0134089. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Immunohistochemical analysis staining SMA or CD68 in paraffin embedded mouse liver tissue sections Samples were incubated with anti-α- SMA (ab7817) or anti-CD68 (ab49777). A Mouse specific HRP/DAB (ABC) Detection IHC Kit (AB64259 was used as a stain and Mayer’s hematoxylin was used as a counterstain. Top panel of images staining CD68 and bottom panel staining α-SMA.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti Mouse HRP/DAB Detection Kit (ab64259)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti Mouse HRP/DAB Detection Kit (ab64259)
    Human colon carcinoma stained with anti p53 antibody using Anti Mouse HRP/DAB Detection Kit (ab64259).

Protocols

  • Mouse specific HRP/DAB detection kit
  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (33)

Publishing research using ab64259? Please let us know so that we can cite the reference in this datasheet.

ab64259 has been referenced in 33 publications.

  • Wisgrill L  et al. Integrative transcriptome analysis deciphers mechanisms of nickel contact dermatitis. Allergy 76:804-815 (2021). PubMed: 32706929
  • Hou QQ  et al. TBC1D3 promotes neural progenitor proliferation by suppressing the histone methyltransferase G9a. Sci Adv 7:N/A (2021). PubMed: 33523893
  • Black EK  et al. The effect of dietary supplementation with high- or low-dose omega-3 fatty acid on inflammatory pathology after traumatic brain injury in rats. Transl Neurosci 12:76-82 (2021). PubMed: 33623714
  • Campelo T  et al. AMPAR-Dependent Synaptic Plasticity Initiates Cortical Remapping and Adaptive Behaviors during Sensory Experience. Cell Rep 32:108097 (2020). PubMed: 32877679
  • Pinto B  et al. Rescuing Over-activated Microglia Restores Cognitive Performance in Juvenile Animals of the Dp(16) Mouse Model of Down Syndrome. Neuron 108:887-904.e12 (2020). PubMed: 33027640
View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

DAB staining of human liver tissues

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
DAB staining of human liver tissues - sample was treated with Ms-ASGP-R Ab. (non-Abcam)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

Submitted Aug 11 2021

Super easy to use, abtained nice image with it!

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
This IHC kit is very easy to use, I get very clear images the first time I use it. It basically has everything you need for IHC staining except mouse primary antibody. Using counter staining will make the image look sharper, please be aware that the kit does not have hematoxylin for nuclear counter staining.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 23 2016

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