Mouse specific HRP/DAB (ABC) Detection IHC Kit (ab64259)
Overview
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Product name
Mouse specific HRP/DAB (ABC) Detection IHC Kit
See all HRP/DAB detection kit kits -
Product overview
Mouse specific HRP/DAB (ABC) IHC Detection Kit ab64259 is a complete kit to detect a primary antibody raised in mouse, using HRP-labeled-streptavidin and a biotinylated anti-mouse secondary antibody.
It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and DAB substrate.
In the method:
- a sample is incubated with an unconjugated primary antibody specific to the target antigen
- a biotinylated secondary antibody then binds to the primary antibody
- HRP-labeled streptavidin then binds to the secondary antibody
- the HRP produces a brown colored substance at the site of primary antibody binding by reacting with DAB. -
Notes
For alternative streptavidin-biotin IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP-DAB IHC kit ab64264
- Anti-Rabbit HRP-DAB IHC Kit ab64261For high sensitivity HRP micro-polymer IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP/DAB Micro-polymer IHC Kit ab236466
- Anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236469
Alternatively, see HRP-AEC IHC detection kits.For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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Tested applications
Suitable for: IHC-Pmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 15 ml 50x DAB Chromogen 1 x 0.5ml Biotinylated goat anti-mouse IgG(H+L) (ab64255) 1 x 15ml DAB substrate 1 x 15ml Hydrogen Peroxide Block 1 x 15ml Protein block 1 x 15ml Streptavidin Peroxidase 1 x 15ml -
Research areas
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Relevance
HRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.
Associated products
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Related Buffer
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent dilution.
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| Notes |
|---|
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IHC-P
Use at an assay dependent dilution. |
Images
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Immunohistochemistry - ab64259Jovicic N et al., PLoS One 10(7). Fig 6D & E. DOI: 10.1371/journal.pone.0134089. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
Immunohistochemical analysis staining SMA or CD68 in paraffin embedded mouse liver tissue sections Samples were incubated with anti-α- SMA (ab7817) or anti-CD68 (ab49777). A Mouse specific HRP/DAB (ABC) Detection IHC Kit (AB64259 was used as a stain and Mayer’s hematoxylin was used as a counterstain. Top panel of images staining CD68 and bottom panel staining α-SMA.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti Mouse HRP/DAB Detection Kit (ab64259)Human colon carcinoma stained with anti p53 antibody using Anti Mouse HRP/DAB Detection Kit (ab64259).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (33)
ab64259 has been referenced in 33 publications.
- Wisgrill L et al. Integrative transcriptome analysis deciphers mechanisms of nickel contact dermatitis. Allergy 76:804-815 (2021). PubMed: 32706929
- Hou QQ et al. TBC1D3 promotes neural progenitor proliferation by suppressing the histone methyltransferase G9a. Sci Adv 7:N/A (2021). PubMed: 33523893
- Black EK et al. The effect of dietary supplementation with high- or low-dose omega-3 fatty acid on inflammatory pathology after traumatic brain injury in rats. Transl Neurosci 12:76-82 (2021). PubMed: 33623714
- Campelo T et al. AMPAR-Dependent Synaptic Plasticity Initiates Cortical Remapping and Adaptive Behaviors during Sensory Experience. Cell Rep 32:108097 (2020). PubMed: 32877679
- Pinto B et al. Rescuing Over-activated Microglia Restores Cognitive Performance in Juvenile Animals of the Dp(16) Mouse Model of Down Syndrome. Neuron 108:887-904.e12 (2020). PubMed: 33027640