Mouse sTNF RI ELISA Kit (TNFRSF1A) (ab202408)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse sTNF RI ELISA Kit (TNFRSF1A)
See all TNF Receptor I kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 1.5% Inter-assay Sample n Mean SD CV% Serum 3 13.8% -
Sample type
Cell culture supernatant, Urine, Serum, EDTA Plasma, Citrate Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.2 pg/ml -
Range
7.81 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 103 101% - 109% Serum 113 107% - 118% Cell culture media 95 94% - 95% EDTA Plasma 102 97% - 109% Citrate Plasma 106 97% - 112% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Rabbit, Goat, Horse, Cow, Dog -
Product overview
Abcam’s sTNF RI (TNFRSF1A) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of sTNF RI protein in mouse serum, plasma, urine and cell culture supernatant.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
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Notes
Tumor necrosis factor receptor superfamily member 1A (TNF RI) is a transmembrane protein with an extracellular domain that binds to TNF alpha. This extracellular domain can be proteolytically cleaved to make soluble TNF RI. The standard protein in this kit is soluble TNF RI and the capture and detector antibodies were raised against this region of TNF RI.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse sTNF RI Capture Antibody 1 x 600µl 10X Mouse sTNF RI Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BC 1 x 6ml Mouse sTNF RI Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. -
Involvement in disease
Familial hibernian fever
Multiple sclerosis 5 -
Sequence similarities
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
Domain
The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP. -
Post-translational
modificationsThe soluble form is produced from the membrane form by proteolytic processing. -
Cellular localization
Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted. - Information by UniProt
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Alternative names
- CD120a
- FPF
- MGC19588
see all -
Database links
- Entrez Gene: 21937 Mouse
- SwissProt: P25118 Mouse
Applications
Our Abpromise guarantee covers the use of ab202408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Mouse sTNF RI ELISA Kit (TNFRSF1A) (ab202408)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse TNFRSF1A standard curve comparison data.Standard curve comparison between mouse TNFRSF1A SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
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Example of sTNF RI standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of sTNF RI in mouse serum, plasma (citrate), and platelet poor plasma (EDTA).The concentrations of sTNF RI were measured in duplicate and interpolated from the sTNF RI standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF RI concentration was determined to be 3,427 pg/mL in mouse serum, 2,484 pg/mL in mouse plasma (Citrate), and 2,982 pg/mL in mouse plasma (EDTA).
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Interpolated concentrations of sTNF RI in mouse urine and L929 stimulated cell culture supernatant.The concentrations of sTNF RI were measured in duplicate and interpolated from the sTNF RI standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF RI concentration was determined to be 1,754 pg/mL in mouse urine, 538.3 pg/mL L929 stimulated cell culture supernatant.
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Comparison of secreted sTNF RI in unstimulated and PMA/PHA-stimulated L929 and J774A.1 Cells.L929 and J774A.1 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. sTNF RI was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and J774A.1 and cell culture media. Measured values were interpolated from the sTNF RI Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed. sTNF RI is undetectable in media.
Protocols
References
ab202408 has not yet been referenced specifically in any publications.
