Mouse Surfactant protein D / SP-D ELISA Kit (ab213890)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 312 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Surfactant protein D / SP-D ELISA Kit
See all Surfactant protein D/SP-D kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 641pg/ml 26.92 4.2% 2 16 2012pg/ml 151.05 7.5% 3 16 9766pg/ml 625.02 6.4% Inter-assay Sample n Mean SD CV% 1 24 618pg/ml 37.69 6.1% 2 24 1957pg/ml 162.43 8.3% 3 24 9758pg/ml 692.81 7.1% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
312 pg/ml - 20000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
The Mouse Surfactant protein D / SP-D Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213890) is designed for the quantitative detection of Mouse Surfactant protein D / SP-D in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A polyclonal antibody from sheep specific for Surfactant protein D / SP-D has been precoated onto 96-well plates. Standards (Expression system for standard: CHO; Immunogen sequence: A20-F374) and test samples are added to the wells, a biotinylated detection polyclonal antibody from sheep specific for Surfactant protein D / SP-D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Surfactant protein D / SP-D amount of sample captured in plate.
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Notes
Surfactant, pulmonary-associated protein D, also known as SFTPD or Surfactant protein D / SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the Surfactant protein D / SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SPD include roles in innate immunity and surfactant metabolism, Surfactant protein D / SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What's more, SP-A and Surfactant protein D / SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Adhesive Plate Seal 4 units 4 units Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-Mouse Surfactant protein D / SP-D coated Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti-Mouse Surfactant protein D / SP-D antibody 1 x 100µl 1 x 100µl Lyophilized recombinant Mouse Surfactant protein D / SP-D standard 2 vials 2 vials Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
Research areas
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Function
Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. -
Sequence similarities
Belongs to the SFTPD family.
Contains 1 C-type lectin domain.
Contains 1 collagen-like domain. -
Post-translational
modificationsThe N-terminus is blocked.
Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates. -
Cellular localization
Secreted > extracellular space > extracellular matrix. Secreted > extracellular space > surface film. - Information by UniProt
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Alternative names
- COLEC 7
- COLEC7
- Collectin-7
see all -
Database links
- Entrez Gene: 20390 Mouse
- SwissProt: P50404 Mouse
- Unigene: 1321 Mouse
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab213890 has been referenced in 1 publication.
- Punsawad C et al. Surfactant Protein D Is Altered in Experimental Malaria-Associated Acute Lung Injury/Acute Respiratory Distress Syndrome. J Trop Med 2019:9281605 (2019). PubMed: 31467567