Overview

  • Product name

    Mouse Surfactant protein D / SP-D ELISA Kit
    See all Surfactant protein D/SP-D kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 2.2ng/ml 0.099 4.5%
    2 16 4.6ng/ml 0.268 5.7%
    3 16 10.5ng/ml 0.672 6.4%
    Inter-assay
    Sample n Mean SD CV%
    1 24 2.4ng/ml 0.139 5.8%
    2 24 5.3ng/ml 0.318 6%
    3 24 12.3ng/ml 0.972 7.9%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    312 pg/ml - 20000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    The Mouse Surfactant protein D / SP-D Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213890) is designed for the quantitative detection of Mouse Surfactant protein D / SP-D in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for Surfactant protein D / SP-D has been precoated onto 96-well plates. Standards (Expression system for standard: CHO; Immunogen sequence: A20-F374) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Surfactant protein D / SP-D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Surfactant protein D / SP-D amount of sample captured in plate.

  • Notes

    Surfactant, pulmonary-associated protein D, also known as SFTPD or Surfactant protein D / SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the Surfactant protein D / SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SPD include roles in innate immunity and surfactant metabolism, Surfactant protein D / SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What's more, SP-A and Surfactant protein D / SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Mouse Surfactant protein D / SP-D coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Mouse Surfactant protein D / SP-D antibody 1 x 130µl
    Lyophilized recombinant Mouse Surfactant protein D / SP-D standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
  • Sequence similarities

    Belongs to the SFTPD family.
    Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.
  • Post-translational
    modifications

    The N-terminus is blocked.
    Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix. Secreted > extracellular space > surface film.
  • Information by UniProt
  • Alternative names

    • COLEC 7
    • COLEC7
    • Collectin-7
    • Collectin7
    • Lung surfactant protein D
    • PSP D
    • PSP-D
    • PSP-D Surfactant protein D
    • PSPD
    • Pulmonary surfactant apoprotein
    • Pulmonary surfactant associated protein D
    • Pulmonary surfactant associated protein PSP-D
    • Pulmonary surfactant-associated protein D
    • SFTP 4
    • SFTP4
    • SFTPD
    • SFTPD_HUMAN
    • SP D
    • SP-D
    • Surfactant associated protein pulmonary 4
    • Surfactant protein D
    • Surfactant pulmonary associated protein D
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Mouse Surfactant protein D / SP-D ELISA Kit (ab213890) Standard Curve.

Protocols

References

ab213890 has not yet been referenced specifically in any publications.

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