Overview

  • Product name

    Mouse Survivin ELISA Kit (Birc5)
    See all Survivin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    NIH/3T3 5 3.1%
    Inter-assay
    Sample n Mean SD CV%
    NIH/3T3 3 13.1%
  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    9 pg/ml
  • Range

    93.75 pg/ml - 6000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 177 172.5% - 181.7%
    Cell culture media 133.3 122.5% - 149.8%
    Heparin Plasma 112.3 105.9% - 121.4%
    Citrate Plasma 90 85.4% - 98.3%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s Survivin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Survivin protein in mouse serum, plasma, cell culture supernatant, cell and tissue extracts.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

    Sensitivity:

    Samples diluted in Sample Diluent NS – 9 pg/mL
    Samples diluted in Sample Diluent 25BP – 47 pg/mL
    Samples diluted in Sample Diluent NBP – 9 pg/mL
    Samples diluted in 1X Cell Extraction Buffer PTR – 25 pg/mL

  • Notes

    Survivin is a multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Survivin is a component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Survivin acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and it participates in the organization of the center spindle by associating with polymerized microtubules. Survivin complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Survivin may counteract a default induction of apoptosis in G2/M phase. The acetylated form of Survivin represses STAT3 transactivation of target gene promoters. Survivin may play a role in neoplasia. Survivin may be an inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 of Survivin do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab202401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • The concentrations of Survivin were measured in duplicates, interpolated from the Survivin standard curve and corrected for sample dilution. Note that 1X Diluted extract samples were at 1 mg/mL. The interpolated, dilution factor-corrected values are plotted (mean +/- SD, n=2).

  • NIH/3T3 cells were cultured in the absence or presence of 200 ng/mL nocodazole for 17 hours. The extracts of these cells (adjusted to the same protein concentration) were measured in dilution series (as indicated) with this kit. Interpolated concentrations of Survivin adjusted for sample dilution are graphed in pg of Survivin per mL of 1X Diluted extract (mean +/- SD, n = 2).

Protocols

References

ab202401 has not yet been referenced specifically in any publications.

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