Key features and details
- Sensitivity: 0.35 ng/ml
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich
- Reacts with: Mouse
Product nameMouse Syndecan-1 ELISA kit
See all Syndecan-1 kits
Intra-assay Sample n Mean SD CV% Various 6.3% Inter-assay Sample n Mean SD CV% Various 7.6%
Sample typeCell culture supernatant, Serum, Plasma, Other biological fluids
Sensitivity< 0.35 ng/ml
Range16 - 0.5
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Predicted to work with: Human
Mouse Syndecan-1 ELISA kit (ab273165) is for the in-vitro qualitative and quantitative determination of Syndecan-1 in cell culture supernatants, buffered solutions or mouse serum, plasma, or other body fluids. This assay will recognize both natural and recombinant mouse Syndecan-1.
A capture antibody highly specific for Syndecan-1 has been coated to the wells of the microtiter strip plate provided during manufacture.
Binding of Syndecan-1 in samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti-Syndecan-1 secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is then removed.
The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing.
A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of Syndecan-1 present in the samples and standards.
The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of Syndecan-1 in any sample tested.
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Antibody diluent 1 x 7ml Biotinylated Antibody Syndecan-1 1 x 400µl HRP Diluent 1 x 12ml Plastic Plate Cover 2 units Standard diluent Buffer 1 x 15ml Stop Reagent 1 x 11ml Streptavidin-HRP 2 x 5µl Syndecan-1 Coated Microwell strips 1 unit Syndecan-1 Standard 2 vials TMB Substrate 1 x 11ml Wash Buffer 1 x 10ml
FunctionCell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.
Sequence similaritiesBelongs to the syndecan proteoglycan family.
- Information by UniProt
- CD138 antigen
ab273165 has not yet been referenced specifically in any publications.