Overview

  • Product name

    Mouse TARC ELISA Kit (CCL17)
    See all TARC/CCL17 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 9.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 6.8%
  • Sample type

    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    2.5 pg/ml
  • Range

    7.8 pg/ml - 500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 86 81% - 92%
    Cell culture media 98 93% - 101%
    Heparin Plasma 81 76% - 84%
    EDTA Plasma 107 103% - 112%
    Citrate Plasma 101 95% - 107%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Human
  • Product overview

    TARC in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TARC protein in mouse serum, plasma and cell culture supernatant.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:
    Samples in Sample Diluent NS: 2.5 pg/mL
    Samples in Sample Diluent 5BS: 3.4 pg/mL
    Samples in Sample Diluent 50BS: 4.6 pg/mL

  • Notes

    TARC (also known as C-C motif chemokine 17) is a chemokine that functions through chemokine receptors CCR4 and CCR8. TARC is expressed in the thymus as well as by dendritic cells, keratinocytes and bronchial epithelial cells.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse TARC Capture Antibody 1 x 600µl
    10X Mouse TARC Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BR 1 x 6ml
    Mouse TARC Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Sample Diluent NBS 1 x 20ml
    Sample Diluent NS 1 x 50ml
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Chemotactic factor for T-lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4.
  • Tissue specificity

    Expressed at high levels in thymus and at low levels in the lung, colon and small intestine.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • A-152E5.3
    • A152E53
    • ABCD 2
    • ABCD2
    • C-C motif chemokine 17
    • CC chemokine TARC
    • CCL17
    • CCL17_HUMAN
    • Chemokine CC Motif Ligand 17
    • MGC138271
    • MGC138273
    • SCYA17
    • Small Inducible Cytokine A17
    • Small Inducible Cytokine A17 Precursor
    • Small Inducible Cytokine Subfamily A (Cys Cys)
    • Small Inducible Cytokine Subfamily A (Cys Cys) Member 17
    • Small-inducible cytokine A17
    • T Cell Directed CC Chemokine
    • Thymus and activation regulated chemokine
    • Thymus and activation-regulated chemokine
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab213474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between mouse TARC SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed. 

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of TARC were measured in duplicates, interpolated from the TARC standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • The concentrations of TARC were measured in duplicates, interpolated from the TARC standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TARC concentration was determined to be 91 pg/mL in serum, 25.4 pg/mL in plasma (citrate), 23.0 pg/mL in plasma (EDTA) and 25.4 pg/mL in plasma (heparin).

  • The concentrations of TARC were measured in duplicates, interpolated from the TARC standard curves and corrected for sample dilution. Undiluted samples are as follows: splenocyte supernatant 50%, and lung supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TARC concentration was determined to be 25 pg/mL in splenocyte supernatant and 756 pg/mL in lung supernatant.

Protocols

References

ab213474 has not yet been referenced specifically in any publications.

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